| 检测副溶血弧菌TDH斑点免疫胶体金渗滤法的研究 |
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| 引用本文： | 杨靖亚,陆晓帆,王珍. 检测副溶血弧菌TDH斑点免疫胶体金渗滤法的研究[J]. 中国免疫学杂志, 2012, 28(8): 733-736,751 |
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| 作者姓名： | 杨靖亚 陆晓帆 王珍 |
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| 作者单位： | 上海海洋大学海洋科学研究院,上海水产品加工及贮藏工程技术研究中心,上海,201306 |
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| 基金项目： | 上海市科委工程中心建设项目(11DZ2280300);上海市教育委员会重点学科建设项目(J50704) |
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| 摘 要： | 目的:制备特异性检测副溶血弧菌TDH的斑点免疫胶体金渗滤测试盒(Dot immunogold filtration assay,DIG-FA)。方法:T6D4和T9H4两株抗体分别标记于金颗粒和点样于硝酸纤维素膜,通过双抗体夹心法来开发斑点免疫胶体金渗滤测试盒,并对测试盒的特异性、重复性、稳定性做了分析。结果:研制的斑点免疫胶体金渗滤测试盒能够特异性检测副溶血弧菌TDH,其最低检测限达到250 ng/ml TDH,该测试盒在4℃恒温条件下保存12周后仍表现出准确和稳定的检测结果。结论:成功制备了检测副溶血弧菌TDH的斑点免疫胶体金渗滤测试盒,对现场快速检测TDH提供了可靠的测试工具。
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| 关 键 词： | 副溶血弧菌 耐热直接溶血毒素 斑点免疫胶体金渗滤法 快速检测 |
Development of dot-immunogold filtration assay to detect TDH produced by Vibrio parahaemolyticus |
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| YANG Jing-Ya , LU Xiao-Fan , WANG Zhen. Development of dot-immunogold filtration assay to detect TDH produced by Vibrio parahaemolyticus[J]. Chinese Journal of Immunology, 2012, 28(8): 733-736,751 |
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| Authors: | YANG Jing-Ya LU Xiao-Fan WANG Zhen |
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| Affiliation: | .Ocean Science Institution Research Institute,Shanghai Ocean University,Shanghai Engineering Center for Processing and Storage of Acquatic Product,Shanghai 201306,China |
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| Abstract: | Objective:To develop a Dot immunogold filtration assay to especially detect TDH(thermostable direct hemolysin) produced by vibrio parahaemolyticus.Methods:T6D4 and T9H4 were coated to nanogold and ejected onto nitrocellulose filter membrane respectively.We developed the test kit via sandwich assay with double-antibodies,and analyzed its specificity,repeatablity and stability.Results:The DIGFA kit could especially detect TDH at the lowest level of 250 ng/ml.It also exhibited exact and stable testing result after 12 weeks later stored under 4℃.Conclusion:The study successfully developed the DIGFA kit,which could be widely applied into detect TDH rapidly on the scene. |
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| Keywords: | Vibrio parahaemolyticus Thermostable direct hemolysin Dot immunogold filtration assay Rapid detection |
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