贵州省不同地区2008年虫媒病毒调查

目的调查贵州省不同地区虫媒病毒分布状况,对采集的蚊虫标本进行病毒分离与鉴定。方法 2008年7月在贵州省黔西、德江、榕江和从江县的4个标本采集点使用诱蚊灯采集蚊虫标本,通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果采集到3属4种共计9160只蚊虫标本,从中分离到9株病毒,鉴定结果显示8株为盖塔病毒,1株为流行性乙型脑炎病毒(JEV);贵州省新分离盖塔病毒与原型株相比,核苷酸同源性为94.5%～94.9%,氨基酸同源性为97.4%～97.6%;新分离JEV与基因Ⅰ型JEV的同源性最高,系统进化分析结果显示属于基因Ⅰ型JEV。结论在贵州省分离到盖塔病毒和基因Ⅰ型JEV。盖塔病毒新分离株与我国其他省份分离株进化关系较近;JEV新分离株与四川省分离株进化关系较近。

Investigation of arboviruses in different regions of Guizhou province,China in 2008

Objective To investigate the distribution profiles of arboviruses in different regions of Guizhou province,China.Methods Mosquito samples were collected using mosquito lamps in Qianxi county,Dejiang county,Rongjiang county,and Congjiang county,Guizhou province in July 2008 and then used for virus isolation by tissue culture.The obtained virus isolates were identified by serological and molecular biological methods.Homology and phylogenetic analysis were performed on viral sequences by bioinformatics.Results A total of 9160 mosquitoes(4 species,3 genera) were collected.Out of the 9 virus strains isolated from the mosquitoes,8 were identified as Getah virus(GETV),and 1 was identified as Japanese encephalitis virus(JEV).Compared with the prototype strain,the GETV strains had a nucleotide homology of 94.5%-94.9% and an amino acid homology of 97.4%-97.6%.The newly isolated JEV belonged to genotype Ⅰ,according to the homology and phylogenetic analysis.Conclusion GETV and genotype Ⅰ JEV were first isolated from mosquitoes collected in Guizhou province,China.The GETV strains in Guizhou province have a close phylogenetic association with the strains isolated from other provinces in China,and the JEV strain in Guizhou province has a close phylogenetic association with the strains isolated from Sichuan province,China.