单纯强迫症和共病双相障碍的强迫症患者5-HTTLPR基因多态性研究

目的:探讨汉族人群中5-羟色胺转运体启动子区(5-HTTLPR)基因多态性与单纯强迫症和共病双相障碍的强迫症之间的相关性。方法:按照美国《精神疾病诊断与统计手册》(DSM-Ⅳ)的强迫症的诊断标准选择单纯强迫症患者(强迫症组)52例、共病双相障碍的强迫症患者(共病组)48例和健康对照者(对照组)57例;每例研究对象抽取静脉血提取DNA,应用聚合酶链式反应(PCR)及扩增片断长度多态技术检测5-HTTLPR基因多态性,采用病例对照的关联分析方法对基因型和等位基因频率进行分析。结果:三组的5-HTTLPR基因型符合Hardy-Weinberg平衡法则;三组间5-HTTLPR基因型和等位基因频率分布均无显著性差异;经性别分层后三组间5-HTTLPR的基因型与等位基因频率也无显著性差异。结论:5-HTTLPR基因型和等位基因频率分布与强迫症和共病双相障碍的强迫症无关联性。

Research of the polymorphisms serotonin transporter promoter region gene in Obsessive-compulsive disorder patients and obsessive-compulsive disorder Comorbid with bipolar disorder patients

Objective:To explore the association between polymorphisms of serotonin transporter promoter region(5-HTTLPR) and the pathogenesis of obsessive-compulsive disorder(OCD) and obsessive-compulsive disorder co morbid with bipolar disorder(BD) in the Han nationality.Methods:52 Patients were recruited according to the diagnosis criteria of OCD in DSM-Ⅳ,and 48 Patients were also recruited according to the diagnosis criteria of bipolar disorder and OCD in DSM-Ⅳ,and 57 healthy persons in the control group;Venous blood was taken from all individuals into the ACD-containing tube,After DNA isolation,the 5-HTTLPR gene determined by the polymerase chain reaction amplification fragment length polymorphism technique.The case-control association analysis was adopted to analyze the frequencies of genotype and alleles between the three groups.Results: The values of the genotypes of the three groups consistent with the Hardy-Weinberg equilibrium;The distribution of genotypes or alleles of 5-HTTLPR genes had no significant differences between the three groups;Even after dividing by gender,there were no significant differences between the three groups.Conclusions: 5-HTTLPR polymorphism is not associated with OCD or OCD comorbid with BD.