辽宁省部分地区2008年虫媒病毒分离鉴定

目的在辽宁省采集蚊虫标本进行病毒分离,了解虫媒病毒分布情况。方法2008年夏季在辽宁省丹东和锦州市采集蚊虫标本,利用C6/36和BHK-21等细胞分离病毒;对新分离病毒利用血清学、分子生物学和生物信息学方法进行鉴定。结果在辽宁省丹东和锦州市共采集蚊虫标本3属5种9296只,包括库蚊属的三带喙库蚊、淡色库蚊,伊蚊属的刺扰伊蚊、背点伊蚊和按蚊属的中华按蚊。从蚊虫标本中共得到4株病毒分离物,其中1株为乙型脑炎(乙脑)病毒(丹东市的三带喙库蚊),2株为版纳病毒(锦州市的中华按蚊),1株为辽宁病毒和版纳病毒混合(锦州市的中华按蚊)。新分离乙脑病毒属于基因Ⅰ型乙脑病毒,E基因编码病毒毒力和抗原表位的位点未发生改变。结论在辽宁省再次分离到乙脑病毒,并首次证明辽宁省存在辽宁病毒。

Arboviruses isolated from mosquitoes in Liaoning province in 2008

Objective To learn the distribution of arboviruses in mosquitoes in Liaoning province.Methods The samples were collected in Dandong and Jinzhou of Liaoning province in 2008.The isolation of viruses was conducted by way of C6/36 and BHK-21 cell culture,with identification of viruses done by serological,molecular and bioinformatics methods.Results A total of 9296 mosquitoes consisting of 5 species from the Culex,Aedes and Anopheles genera were trapped.Four virus isolates were obtained from pooled mosquitoes and were identified as Japanese encephalitis virus(JEV)(from Cx.tritaeniorhynchus),Banna virus(2 isolate from An.sinensis) and the mixture of Liaoning virus and Banna virus(from An.sinensis).Genotype of JEV isolate belonged to genotypeⅠ and no mutation of amino acid sequence coding conformational epitope occurred in Envelop protein.Conclusion GenotypeⅠ JEV was isolated again in Liaoning province and Liaoning virus was documented in this area for the first time.