血管活性肠多肽及降钙素基因相关肽对大鼠腹腔肥大细胞组胺释放的作用

目的:研究血管活性肠多肽(Vasoactive intestinal peptide,VIP)及降钙素基因相关肽(Calcitonin gene relatedpeptide,CGRP)对大鼠腹腔肥大细胞脱颗粒的诱导作用;了解神经多肽与肥大细胞的相互关系。方法:分离、纯化SD大鼠腹腔肥大细胞;应用不同浓度的VIP和CGRP作用于大鼠腹腔肥大细胞后,同位素放射液态闪烁法检测肥大细胞的组胺释放、45Ca摄入的变化;同时观察大鼠腹腔肥大细胞经5×10-6mol/L VIP受体抑制剂L-8-K处理后,对VIP诱导脱颗粒作用的影响。结果:5×10-6mol/L的VIP作用后大鼠腹腔肥大细胞组胺释放及45Ca摄入明显增加,并且这种变化与VIP呈剂量效应关系;CGRP对大鼠腹腔肥大细胞组胺释放无诱导作用;L-8-K作用后,肥大细胞对VIP的诱导活化作用无改变。结论:VIP可引起肥大细胞钙内流增加,进一步诱导肥大细胞脱颗粒、释放组胺等生物活性物质,产生生物学效应;这种作用是受体非依赖性的,且与VIP的分子构型有关。

Histamine release of rat peritoneal mast cells induced by vasoactive intestinal peptide and calcitonin gene related peptide

Objective:To investigate the inducing role of VIP and CGRP on degranulation of rat peritoneal mast cells(RPMCs),and to understand the relationship between the neuropeptides and mast cells.Methods:The SD Rat Peritoneal Mast cells(RPMCs) were isolated,purified and dispensed.Isotope liquid scintillation counting was used to measure the content of histamine release,45Ca influx of the RPMCs which were treated by different concentration of VIP and CGRP;at the same time,L-8-K preprocessed RPMCs were used to observed weather the VIP receptor-inhibitor could interfere VIP with inducing degranulation of RPMCs.Results:After the RPMCs were treated with 5×10-6 mol/L VIP,the histamine release and 45Ca influx of the RPMCs were increased,remarkably;and these changes were dose-dependent with VIP.And there was no effect of CGRP on histamine release and 45Ca influx of the RPMCs;L-8-K could not interact with the inducing role of VIP on histamine release and 45Ca influx of the RPMCs.Conclusion:VIP could induce degranulation and histamine release from RPMCs by calcium influx increase pathway,independent of VIP receptors;and the RPMC degranulation is related with the number of basic amino acid remnant and molecular structure of the neuropeptide molecule.