普罗布考、氯吡格雷、阿托伐他汀综合疗法对兔动脉粥样硬化相关炎性因子的抑制作用

目的探讨普罗布考、氯吡格雷和阿托伐他汀联合用药对兔动脉粥样硬化相关炎性因子的抑制作用。方法新西兰雄性大白兔36只随机分为4组,分别给予正常及高脂饮食,6周后高脂组再分为高脂组、高脂 阿托伐他汀组、高脂 普罗布考、氯吡格雷、阿托伐他汀联合干预组,继续喂养4周后采用免疫组织化学染色法观察主动脉斑块部位炎性因子的表达。结果高脂组主动脉中血管细胞粘附分子1、白细胞介素6和单核细胞趋化蛋白1的表达明显增加。阿托伐他汀组炎性因子血管细胞粘附分子1、白细胞介素6和单核细胞趋化蛋白1的表达下降,与高脂组比较差异具有显著性(P<0.05),联合干预组血管细胞粘附分子1、白细胞介素6和单核细胞趋化蛋白1的表达下降更为明显(P<0.01),与阿托伐他汀组比较差异具有显著性(P<0.05)。结论抗氧化、抗血小板、他汀联合用药对动脉粥样硬化炎性因子的表达具有明显的抑制作用,其作用优于单纯使用阿托伐他汀。

Effects of Probulol Clopidogrel Atorvastatin United Therapy on Markers of Inflammation of Rabbit Atherogenesis

Aim To study the effect of united therapy of probucol(P),clopidogrel(Anti-thrombosis,A) and atorvastatin(statin,S) on Markers of inflammation of rabbit atherogenesis. Methods 36 rabbits were randomized into four groups. One group did not receive treatments and served as control group. Atherosclerosis was induced in the aorta arteries of rabbits by atherogenic diet in the rest groups for 6 weeks. Then,animals were randomized to receive treatment or no treatment. One group of treatment were received atorvastatin,and the other accepted probucol and clopidogre and atorvastatin and killed after 4 weeks. Results Arterial macrophage infiltration was abolished by the treatment of atorvastatin and united group. Vascular cell adhesion molecule-1(VCAM-1) and interleukin-6(IL-6)were significantly diminished in the neointima and the media,and the same were monocyte chemoattractant protein-1(MCP-1). Notedly,there was a more evident effect in united therapy than Atorvastatin in anti-inflammation. Conclusions Atorvastatin and united therapy diminishes the neointimal inflammation in rabbit atherosclerosis model,and the effect of united therapy was more than atorvastatin. This could contribute to the more stabilization of the atherosclerotic plaque,and may be a more meaningful additional prediction for the reduction of acute ischemic events in patients treated with united therapy.