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| 人胚胎成纤维细胞对人胚胎干细胞生长的作用 | |
| 引用本文： | 徐令,黄绍良,李树浓,吴旋. 人胚胎成纤维细胞对人胚胎干细胞生长的作用[J]. 中国病理生理杂志, 2001, 17(1): 1-4 |
| 作者姓名： | 徐令 黄绍良 李树浓 吴旋 |
| 作者单位： | 1. 中山医科大学孙逸仙纪念医院儿科, 广东 广州 510120; 2. 中山医科大学病理生理教研室, 广东 广州 510089; 3. 第三军医大学生理教研室, 重庆400038 |
| 基金项目： | 国家自然科学基金资助(No.39800134);广东省自然科学基金资助(No.980687) |
| 摘 要： | 目的：比较人和小鼠胚胎成纤维细胞对人胚胎干细胞生长的作用，为胚胎干细胞定向诱导各系统细胞应用于临床，消除异种蛋白污染打下基础。方法：分别采用人胚胎成纤维细胞和小鼠胚胎成纤维细胞为饲养层细胞，支持人受精卵的培养，观察其增殖和分化情况。结果：人和小鼠胚胎成纤维细胞分别加入白血病抑制因子（hLIF）均能很好支持人胚胎干细胞生长增殖，并保持未分化状态。结论：完全可以使用人胚胎成纤维细胞支持人胚胎干细胞增殖，消除异种蛋白污染的可能性，为胚胎干细胞定向诱导分化发育应用于临床打下坚实基础。 |
| 关 键 词： | 胚胎 干细胞 成纤维细胞 人类 |
| 文章编号： | 1000-4718(2001)01-0001-04 |
| 收稿时间： | 1999-09-22 |
| 修稿时间： | 1999-09-22 |
Effects of human/mouseembryonic fibroblast cells on the growth of human embryonic stem cells |
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| XU Ling ,HUANG Shao-liang ,LI Shu-nong ,WU Xuan. Effects of human/mouseembryonic fibroblast cells on the growth of human embryonic stem cells[J]. Chinese Journal of Pathophysiology, 2001, 17(1): 1-4 | |
| Authors: | XU Ling HUANG Shao-liang LI Shu-nong WU Xuan |
| Affiliation: | 1. Pediatric Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China; 2. Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou510089, China; 3. Department of physiology, the Third Military Medical University, Chongqing 400038, China |
| Abstract: | AIM: In order to replace mouse embryonic fibroblast cells by human embryonic fibroblast cells to support the growth of human embryonic stem cells, the effects of human/mouse embryonic fibroblast cells on the growth of human embryonic stem cells were compared. METHODS: Both mouse and human embryonic fibroblast cells were used as feeder layer to support human embryonic stem cells. The proliferation and differentiation of human embryonic cells were observed. RESULTS: Combined use of human leukemia inhibitory factor with human/mouse feeder layer cells would support growth and proliferation of human embryonic stem cells and kept in undifferentiated condition. There was no difference between human/mouse cell. CONCLUSION: Human embryonic fibroblast cells can be used to support proliferation of human embryonic stem cells, eliminating the influence of foreign protein. |
| Keywords: | Embryo Stem cells Fibroblasts Human |
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