减少自体游离脂肪移植吸收的实验研究

自体脂肪游离移植在美容整形外科中广泛应用。但其后期过度吸收问题一直未能得到解决。本实验将碱性成纤维细胞生长因子（ｂＦＧＦ）应用于脂肪游离移植，观察其对移植脂肪的生存与保持的作用，以期获得减少移植脂肪过度吸收的途径。实验采用ＳＤ雄性大鼠１６只，分两组：Ａ组，面部右侧为实验侧，植入加有以５０００ｍｇ％纤维蛋白原为缓释剂的ｂＦＧＦ的小颗粒脂肪；左侧为对照侧，植入单纯小颗粒脂肪。Ｂ组，右侧为实验侧，植入同Ａ组实验侧；左侧为对照侧，显微血管吻合脂肪移植。术后３个月结果显示：显微血管吻合移植脂肪重量改变最小，组织学观察基本正常。单纯脂肪游离移植重量变化最大，镜下存在大量囊状脂池、坏死区及纤维化。ｂＦＧＦ有利于游离移植脂肪存活，减少移植组织的吸收。

A study on lessening resorption in autologous free fat transplantation

Autologous free fat transplantation has widely been applied in the field of aesthetic plastic surgery for a long time. However,the problem of over absorption of fat has still not been solved.An experiment was designed tosolve transplantation and observing its effect on tissue maintenance.Sixteen male SD rats,as experimental models were divided into two groups:1) Pearl fat graft with bFGF in 5 000 mg% fibrinogen in the right lateral facial area versus pearl fat graft in the left .2)Pearl fat graft with bFGF in 5 000 mg % fibrinogen in the right lateral facial area versus free fat flaps by reanastomosed in the left cervicofacial region.Postoperative 3 months,free flaps by microsurgery exhibited the least change in weight and showed normal histologic appearance.Conversely,the biggest change in weight occurred in the free fat graft alone,and it showed microscopically a great number of cystlike cavities,focal necrosis and fibrotic tissue. A change of weight in the free fat graft with bFGF was between the two mentioned above. Histologically,it was characterized by irregular size of adipicytes and they were surrounded by some fibrotic tissue.The result suggests that bFGF favor survival and lessen resorption of free fat graft.