| 基于溶胶凝胶碳糊固定化酶的新型电流式葡萄糖传感器测定葡萄糖含量的价值分析 |
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| 引用本文： | 习霞,明亮,刘杰.基于溶胶凝胶碳糊固定化酶的新型电流式葡萄糖传感器测定葡萄糖含量的价值分析[J].南通医学院学报,2006,26(3):160-162. |
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| 作者姓名： | 习霞 明亮 刘杰 |
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| 作者单位： | 1. 南通大学化学化工学院
2. 南通大学神经再生重点实验室 |
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| 基金项目： | 江苏省教育厅自然科学基金 |
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| 摘 要： | 目的:研究用溶胶凝胶固定化酶构建电流式葡萄糖传感器及其性能。方法:用硅溶胶凝胶技术修饰电化学铂化处理的碳粉与葡萄糖氧化酶混合制成的碳糊电极,构成新型电流式葡萄糖传感器,并用电流分析法研究其对葡萄糖的响应。结果:该传感器在电位0V(相对于Ag/AgC l电极)对葡萄糖产生还原电流,在一定浓度范围呈现良好线性关系,具有理想选择性和稳定性。结论:该传感器可用于分析包括生物体液在内的各种样品中葡萄糖含量。
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| 关 键 词： | 葡萄糖传感器 溶胶凝胶 碳糊 固定化酶 电流分析 |
| 文章编号： | 1000-2057（2006）03-0160-03 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006年2月20日 |
A novel design of glucose biosensor: the sol- gel carbon Paste immobilized enzyme electrode |
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| XI Xia,MING Liang,LIU Jie.A novel design of glucose biosensor: the sol- gel carbon Paste immobilized enzyme electrode[J].ACTA Academiae Medicinae Nantong,2006,26(3):160-162. |
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| Authors: | XI Xia MING Liang LIU Jie |
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| Institution: | Nantong University, Nantong226001 |
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| Abstract: | Objective:To develop an amperometric glucose biosensor based on the sol-gel carbon paste immobilized enzyme.Methods:Amperometry was employed to examine the electrochemical respondence of our developed glucose biosensor on additions of glucose.Results:Cathodic currents were induced at the glucose biosensor upon successive additions of glucose at the operating potential of 0V(vs.Ag/AgCl), generating a linearity for the concentration dependence of cathodic currents.Furthermore,the biosensor also showed good selectivity and store stability.Conclusion:Our developed glucose biosensor can be used for monitoring the glucose concentration in biological fluids. |
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| Keywords: | Glucose biosensor Sol-gel Carbon paste Immobilization of enzyme Amperometry |
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