乳腺癌患者T细胞亚群与Ag-NORs定量分析的临床意义

目的：探讨乳腺癌患者的免疫功能变化及临床意义。方法：采用单克隆抗体直接免疫荧光染色法及KI一型肿瘤免疫图像分析仪,对131例乳腺癌患者和37例正常对照组分别测定其外周血T细胞亚群、核仁银染区面积/细胞核面积比值（I.S%）。结果：乳腺癌患者CD3、CD4、CD4/CD8比值,I.S%值比对照组明显降低（P〈0.01）;而CD20、CD8比对照组明显升高。结论：乳腺癌患者免疫功能低下;联合检测T细胞亚群与Ag-NORs可较全面反映乳腺癌患者的免疫功能状态。对了解病情、指导治疗有一定的作用。亦可用T淋巴细胞核仁区嗜银蛋白含量测定替代昂贵的T淋巴细胞亚群检测来评价乳腺癌患者的免疫功能状态。

Clinical Significance of Quantitative Analysis of T Cell Subgroup and Ag-NORs in Patients with Breast Cancer

Objective：To investigate the immunologic dysfunction and clinical significance in patients with breast cancer.Methods：To use the fluorophotometric firect measurement method and the ordinary silver staining technology to separately measures its T cell subgroup and Ag-NORs in 131 example patients with breast cancer and 37 normal control groups.Results：In patients with breast cancer and 37 normal control groups：CD3,CD4,The CD4/CD8 ratio,Ag-NORs in patients with breast cancer were significantly lower than those in the control group（P〈0.01）;But CD20,CD8 were significantly higher than those in the cortrol group（P〈0.01）.Conclusion：Patients with breast cancer：immunologic function is low;To examine the T cell subgroup and Ag-NORs may understand the patients with breast cancer immunologic function state.There is usable in finding out and guidance treatment.Also the content measurement of available Ag-NORs substituted the expensive T lymphocyte subgroup examination evaluates the immunologicfunction state of patients with breast cancer.