恶性胸腹水应用蛋白芯片肿瘤标志物检测的临床价值

目的探讨蛋白芯片技术检测多肿瘤标志物对良恶性胸腹水的鉴别诊断价值。方法检测108例患者的胸腹水癌胚抗原（CEA）、甲胎蛋白（AFP）、癌抗原（CA125）、癌抗原（CA15—3）、糖链抗原（CA19—9）、糖链抗原（cA242）、前列腺特异性抗原（PSA、f-PsA）、神经元特异性烯醇化酶（NSE）、人绒毛膜促性腺激素（hCG、pHCG）、生长激素（HGH）、铁蛋白（Ferritin、Fe）水平，对检测数据进行统计学分析。结果良恶性组标本的胸腹水NSE、PSA／f-PSA比较差异无统计学意义（尸〉O．05），而另外9种肿瘤标志物比较差异有统计学意义（P〈O．05）。胸腹水多肿瘤标志物蛋白芯片检测系统在鉴别良恶性胸腹水诊断中的敏感性、特异性、准确性均高于常规检验。结论检测胸腹水多肿瘤标志物有助于良恶性胸腹水鉴别诊断。

The clinical value of tumor markers in diagnosing malignant hydrothorax and ascites by using protein chip technology

Objective To evaluate the clinical value of multiple tumor markers in differential diagnosis of benign and malignant hydrothorax and ascites by using protein chip technology. Methods The levels of 12 tumor markers, including carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), CA125, CA15-3, carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 242 (CA242),prostate specific antigen (PSA, f-PSA), neurone specific enolase (NSE), human chorionic gonadotrophin (hCG, β-HCG), human growth hormone (HGH), ferroprotein (Ferritin, Fe), were measured in bydrothorax and ascites samples from 108 cases of patients, and the date were statistically analyzed.Results There were no significant differences in NSE and PSA/f-PSA levels in hydrothorax and ascites between the benign group and the malignant group (P＞0.05), but there were in levels of other nine tumor markers (P＜0.05). The sensitivity, specificity and accuracy of multiple tumor markers were significantly higher than those of routine test in differential diagnosis of benign and malignant hydrothorax and ascites. Conclusion The detection of multiple tumor markers contributes to differential diagnosis of benign and malignant hydrothorax and ascites.