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| 高效液相色谱法测定小儿豉翘清热颗粒中栀子苷含量 | |
| 引用本文： | 莫遗盛,许海棠,王冬梅,黄贵明. 高效液相色谱法测定小儿豉翘清热颗粒中栀子苷含量[J]. 中国药业, 2008, 17(17): 21-22 |
| 作者姓名： | 莫遗盛 许海棠 王冬梅 黄贵明 |
| 作者单位： | 1. 广西医科大学第三附属医院药剂科,广西,南宁,530031 2. 广西大学化学化工学院,广西,南宁,530001 3. 广西医科大学制药厂,广西,南宁,530021 |
| 摘 要： | 目的建立测定小儿豉翘清热颗粒中栀子苷含量的高效液相色谱（HPLC）法。方法采用Waters C18柱（250mm×4．6mm，5um），流动相为乙腈-水（15：85），流速为1．0mL／min，检测波长为238nm，柱温为25℃。结果栀子苷质量浓度在0．41～20．5ug／mL范围内与峰面积呈良好的线性关系（r=0．9994），平均回收率为98．13％，RSD为1．18％（n=6）。结论HPLC法简便、快速、准确，适用于小儿豉翘清热颗粒中栀子苷的含量测定。 |
| 关 键 词： | 小儿豉翘清热颗粒 栀子苷 高效液相色谱法 含量测定 |
Determination of Geniposide in Child Chiqiao Qingre Granules by HPLC |
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| Mo Yisheng,Xu Haitang,Wang Dongmei,Huang Guimin. Determination of Geniposide in Child Chiqiao Qingre Granules by HPLC[J]. China Pharmaceuticals, 2008, 17(17): 21-22 | |
| Authors: | Mo Yisheng Xu Haitang Wang Dongmei Huang Guimin |
| Affiliation: | Mo Yisheng, Xu Haitang, Wang Dongmei, Huang Guimin( 1. Department of Pharmac); Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China 530031; 2. College of Chemistry and Chemical Engineering, Guangxi University Nanning, Guangxi, China 530001; 3. Pharmaceutical Factory Guangxi Medical University Nanning, Guangxi, China 530021) |
| Abstract: | Objective To establish a HPLC method for the determination of geniposide in Child Chiqiao Qingre Granules. Methods Waters C18 column （250 mm ×4.6 mm,5 um） was used with aeetonitrile-water （15：85） as the mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was 238 nm. The column temperature was 25 ℃. Results The linearity of geniposide was in the range of 0.41 - 20. 5 ug/mL（ r = 0. 999 4）. The mean recovery rate was 98. 13% and RSD was 1. 18% （ n = 6）. Conclusion This method is simple, rapid and accurate. It can be used as the quality control of Child Chiqiao Qingre Granules. |
| Keywords: | Child Chiqiao Qingre Granules geniposide HPLC content determination |
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