完全睡眠剥夺对大鼠caspase-3表达的影响

目的:探讨完全睡眠剥夺对大鼠caspase-3的影响及其导致记忆、认知等功能障碍的机制.方法:取Sprague-Dawley大鼠35只,随机分为正常对照组、大平台对照组和完全睡眠剥夺组.其中正常对照组(NC)5只大鼠,正常笼养;大平台对照组(TC)只有72、96及120 h 3个时间点,每个时间点5只大鼠;完全睡眠剥夺组(TSD)也有72、96及120 h 3个时间点,每个时间点5只大鼠.采用小平台水环境法建立完全睡眠剥夺模型,在不同的时点处死大鼠后运用免疫组化SP法检测各组大鼠海马和皮质caspase-3的分布规律和动态变化.结果:caspase-3在各组的海马和皮质均有表达,随着睡眠剥夺时间的延长,caspase-3的表达逐渐增加,与正常对照组、大平台对照组差异有显著性(P<0.01).结论:完全睡眠剥夺能引起大鼠海马和皮质caspase-3的表达增加.并随睡眠剥夺时间的延长而渐趋明显.可能是完全睡眠剥夺引起大鼠神经元调亡,进而引起大鼠记忆、认知等功能障碍的机制之一.

Effect of total sleep deprivation on expression of caspase-3 in cortex and hippocampus

Objective:To investigate the effect of total sleep deprivation on expression of caspase-3 in rat crotex and hippocampus.Methods:Twenty-five adult male Sprague-Dawley rats were divided randomly into normal control group(n=5),tank control group(n=5)and total sleep deprivation group(n=15).The animals in TSD group were sacrificed at the following time-points 72 h,96 h and 120 h after TSD, respectively.By strept avidin immunostaining,the expression and distilbution of caspase-3 were detected in the cortex and hippocampus.Results:After TSD 72h,the expression of caspase-3 in TSD group in rat cortex and hippocampus was higher than that in normal control group and tank control group(P<0.01),with prolonged deprivation time,the numbers of immuno-positive cell increased obvously.Later time point subgroup was compared with the former subgroup,with evident statistics significance(P<0.01).Conclusion:Total sleep deprivation might increase the expression of caspase-3 in rat cortex and hippocampus.The result suggests that caspase-3 might be one of neurobiological alterations leading to brain dysfunction such as learn and memory disturbance.