荧光定量PCR检测产妇乳汁中HBV-DNA结果分析

目的:研究分析乙型肝炎携带产妇乳汁中HBV -DNA与血清HBV标志物之间的关系以及探讨母乳喂养安全性问题。方法:采用荧光定量PCR技术检测其12 4例孕期检测有一项以上乙肝血清学指标(HBVM)阳性,其它产检项目正常的产妇,及2 0例乙肝标志物阴性的健康产妇作对照,进行初乳中的HBV -DNA与血清乙肝标志物的检测结果的比较。结果:12 4例HBVM阳性产妇中有72例检出HBV -DNA(5 8.0 % )而对照组无一例阳性。HbeAg阳性组产妇初乳排毒率明显高于HbeAg阴性组,有显著性差异(P <0 .0 1)结论:荧光定量PCR检测乳汁中HBV -DNA结果准确灵敏,可及时反映乙肝携带产妇是否可以母乳喂养,因此我们不可忽视乳汁中HBV -DNA的检测。

The Analyse of the Detection of HBV-DNA in the milk of Parturients by the Fluorescent Quantitative PCR Technique

Objective To see the relation between the HBV marker in the serum and the HBV-DNA in the milk of the parturients who took the hepatitis B,and to study the safety of breast feeding. Methods:To detect 124 milk of the parturients who only had one or above positive HBVM in the prenatal examination by the fluorescent quantitative PCR technique,and took 20 samples of the healthy parturients whose HBVM is negative as the control group. Results:pin the 124 cases,72 found HBV-DNA(58.0%),but none found in the control group. And the colostrums expellent rate of the parturients whose HBeAg is positive is obviously higher than those whose HbeAg is negative, the two groups have significant difference(P<0.01).Conclusions:To detect HBV-DNA in the milk by the fluorescent quantitative PCR technique,the result is accurate and sensitive,it can tell you in time whether the pregnant woman can take bleast feeding or not.So we cannot ignore the detection of the HBV-DNA in the milk of parturients.