| 阿糖胞苷上调白血病细胞CD86分子及细胞因子表达的研究 |
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| 引用本文： | 范冬梅,杨铭,贾海荣,高瀛岱,王金宏,纪庆,熊冬生,杨纯正. 阿糖胞苷上调白血病细胞CD86分子及细胞因子表达的研究[J]. 细胞与分子免疫学杂志, 2008, 24(6): 550-553 |
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| 作者姓名： | 范冬梅 杨铭 贾海荣 高瀛岱 王金宏 纪庆 熊冬生 杨纯正 |
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| 作者单位： | 中国医学科学院北京协和医学院血液学研究所,血液病医院实验血液学国家重点实验室,天津,300020 |
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| 摘 要： | 目的:观察阿糖胞苷(Ara-C)对急性白血病细胞CD86分子表达的影响,并探讨其作用机制.方法:流式细胞术(FCM)检测U937、HL60、NB4细胞经Ara-C处理前后CD86分子表达变化,RT-PCR检测Ara-C对各组细胞CD86mRNA、NF-кB以及细胞因子IFN-γ的表达变化.结果:经Ara-C处理的急性白血病细胞CD86分子表达与对照组相比均明显升高(P<0.05);CD86 mRNA表达水平也明显增强;Ara-C处理后细胞核内NF-кB表达明显上调;并且IFN-γ mRNA在T细胞活化72 h可检测到.结论:Ara-C能使U937、HL60、NB4急性白血病细胞CD86表达增加,有利于NF-кB等转录因子活化,促进CD86转录增强、表达增加并可有效地增强肿瘤细胞的免疫原性,激活T细胞,B7-2在T细胞活化中起着更重要的作用.
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| 关 键 词： | 阿糖胞苷 NF-кB CD86 T细胞 细胞因子 阿糖胞苷 急性白血病细胞 细胞因子表达 分子表达 研究 leukemia cells acute expression 激活 免疫原性 肿瘤细胞 转录因子 细胞活化 可检测 细胞核 增强 表达水平 mRNA 对照组 结果 |
| 文章编号： | 1007-8738(2008)06-0550-04 |
| 修稿时间： | 2007-10-08 |
Up-regnlation of CD86 and cytokine expression in acute leukemia cells by Ara-C |
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| FAN Dong-mei,YANG Ming,JIA Hai-rong,GAO Ying-dai,WANG Jin-hong,JI Qing,XIONG Dong-sheng,YANG Chun-zheng. Up-regnlation of CD86 and cytokine expression in acute leukemia cells by Ara-C[J]. Chinese journal of cellular and molecular immunology, 2008, 24(6): 550-553 |
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| Authors: | FAN Dong-mei YANG Ming JIA Hai-rong GAO Ying-dai WANG Jin-hong JI Qing XIONG Dong-sheng YANG Chun-zheng |
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| Affiliation: | State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academic of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. |
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| Abstract: | AIM: To observe the effects of Ara-c on the expression of CD86 molecule on acute leukemia cells and explore the possible mechanisms. METHODS: The expression of CD86 on U937, HL-60 and NB4 cell lines treated with or without Ara-C wa assayed by flow cytometry. The mRNA expression of CD86, NF-kappaB, IFN-gamma was examined by semiquantitative RT-PCR. RESULTS: UP-regulation of CD86 was observed on those cells treated by Ara-c. The leve of CD86 and NF-kappaB mRNA in Ara-c treated cells was significantly enhanced. IFN-gamma was detectable 72 hours after T cell activation. CONCLUSION: Ara-C can enhance CD86 and NF-kappaB expression on acute leukemia cells, which may play critical role in T cell activation and differentiation. |
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