| 热变性和有机溶剂沉淀联合法提纯超氧化物歧化酶 |
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| 作者单位： | Dept. of Biochem、School of Basic Med. Sci. |
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| 摘 要： | 以抗凝马血为原料，分三组分别采用热变性法、有机溶剂沉淀法及热变性与有机溶剂沉淀联合法对超氧化物歧化酶进行分离提纯，对三组方法所得的粗酶和经ＤＥＡＥ－３２层析后的纯酶分别进行蛋白浓度、酶活力等检测，综合结果与分析表明，热变性与有机溶剂沉淀联合法是提纯ＳＯＤ的优选方法。
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| 关 键 词： | 超氧化物歧化酶，酶活力，比活 |
SOD purified by method of combining organic solvents and heating |
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| Ma Yingzhe,Cao Wenhua,Lu Yanjuan,et al. SOD purified by method of combining organic solvents and heating[J]. Journal of Jilin University: Med Ed, 1996, 0(5) |
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| Authors: | Ma Yingzhe Cao Wenhua Lu Yanjuan et al |
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| Abstract: | |
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| Keywords: | SOD enzyme activity specific activity |
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