Colorimetric assay of urinary 5-hydroxy-3-indoleacetic acid |
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| Authors: | Z K Shihabi E L Wilson |
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| Affiliation: | Clinical Chemistry Laboratory, Department of Pathology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103, USA |
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| Abstract: | We describe a method for 5-hydroxy-3-indoleacetic acid in urine based on its reaction with nitrosonaphthol in the presence of mercaptoethanolamine, an odorless compound. Mercaptoethanolamine shifts the color maximum from 540 nm to 640 nm, intensifies the color and discharges the color of interfering substances. The method is rapid and free from interferences. |
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