脑梗塞患者红细胞补体受体1活性及其数量基因多态性

为探讨脑梗塞(CI)患者红细胞表面补体受体1(ECR1)活性及CR1数量基因表型分布,分别从细胞及基因2个水平揭示了脑梗塞ECR1活性下降的机理,并为基因重组产物补体受体(rCR1)用于早期预防和治疗CI提供理论基础.用红细胞-酵母菌花环结合试验测定CI组及对照组ECR1活性,测定参数为C3bRR(C3b受体花环率)、CICRR(免疫复合物花环率).用聚合酶链反应&&限制性片段长度多态性(PCR-RFLP)方法测定CI组及对照组的数量基因多态性各表型分布情况.与对照组相比,CI组C3bRR下降、CICRR上升,差异均有显著意义(P<0.05).CR1数量基因表型分布CI组与对照组相比,HH型下降,HL增高,差异有显著意义(P<0.05).结果表明CI患者ECR1活性下降与CR1基因表型分布有关.

The Erythrocyte Complement Receptor 1 Activity and CR1 Genomic Polymorphism in the Patients with Cerebral Infarction

The erythrocyte complement receptor 1 (ECR1) activity and CR1 quantitative genotype distribution were studied and the mechanisms of decreased ECR1 activity in cerebral infarction revealed. By using red blood cell yeast rosette test ECR1 activities were measured and by using PCR-RFLP CR1 Hind Ⅲ genomic polymorphism detected in the patients with cerebral infarction and healthy controls. The results showed that the level of C3bRR was decreased and the level of CICRR increased in the patients with cerebral infarction as compared with healthy controls (both P<0.05). CR1 quantitative genotype distribution in the patients with cerebral infarction was differed significantly from that of healthy controls (P<0.05). It was concluded that the decrease of ECR1 activity in the patients with cerebral infarction was correlated with CR1 Hind Ⅲ genomic polymorphism.