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栽培滇龙胆的化学成分研究
引用本文:朱卫萍,赵磊,张国华,饶高雄.栽培滇龙胆的化学成分研究[J].云南中医学院学报,2010,33(5):8-12.
作者姓名:朱卫萍  赵磊  张国华  饶高雄
作者单位:1. 云南中医学院中药学院,云南昆明,650500
2. 云南中医学院中药学院,云南昆明,650500;云南省农业科学院药用植物研究所,云南昆明,650231
基金项目:云南省发改委产业关键技术开发专项"滇龙胆的人工驯化及规范化种植关键技术研究与示范" 
摘    要:目的:研究栽培滇龙胆(Gentiana rigescens Franch.)的化学成分,比较栽培品和野生品化学成分的异同,评价栽培滇龙胆药材品质。方法:用色谱法分离化学成分,根据波谱分析鉴定化学结构;用薄层色谱(TLC)和高效液相色谱(HPLC)进行化学成分的比较分析。结果:从栽培滇龙胆药材中分离鉴定了19个成分;栽培品的TLC及HPLC谱图和野生品一致,龙胆苦苷的含量分别为4.31%(栽培品)和4.71%(野生品)。结论:首次研究了栽培滇龙胆的化学成分,并明确其所含化学成分类型、主要有效成分和野生滇龙胆一致;滇龙胆栽培品和野生品中龙胆苦苷含量相当且均高于法定标准;滇龙胆人工栽培是成功的,应大力发展规模种植。

关 键 词:滇龙胆  栽培  化学成分  含量测定  品质评价

Chemical Constituents of Cultivated Gentiana Rigescens Franch.Based on Latent Endogenous Toxicity Theory
ZHU Wei-Ping,ZHAO Lei,ZHANG Guo-hu,RAO Gao-Xiong.Chemical Constituents of Cultivated Gentiana Rigescens Franch.Based on Latent Endogenous Toxicity Theory[J].Journal of Yunnan College of Traditional Chinese Medicine,2010,33(5):8-12.
Authors:ZHU Wei-Ping  ZHAO Lei  ZHANG Guo-hu  RAO Gao-Xiong
Institution:ZHU Wei-Ping1,ZHAO Lei1,ZHANG Guo-hua1,RAO Gao-Xiong1,2(1.College of Pharmacy,Yunnan university of TCM,Kunming Yunnan 650500,China,2.Institute of Medicinal Plant,Yunnan Academy of Agriculture Science,Kunming Yunnan 650231,China)
Abstract:Objective: To study chemical constituents of cultivated Gentiana rigescen and to appraise the qualities of cultivated species by means of comparison on chemical substance with that of the wild species.Method: The chemical constituents were isolated by chromatography and their structures were identified by spectroscopic analysis.The comparison on chemical substance of cultivated and wild Gentiana rigescens was carried out with TLC and HPLC analysis.Results: Nineteen compounds,including main component gentiop...
Keywords:Gentiana rigescens Franch    Planting  Chemical constituents  Qualities appraisement  
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