千斤藤碱对头颈部恶性肿瘤细胞诱导分化的研究

为观察千斤藤碱（Ｓｔｅｐｈｏｌｉｄｉｎｅ，ＳＴ）对头颈部恶性肿瘤细胞株诱导分化作用，采用ＡＢＣ免疫组化结合图象分析仪及流式细胞仪，以维甲酸（ｒｅｔｉｎｏｉｃａｃｉｄ，ＲＡ）作对照，通过细胞形态学、细胞增殖动力学的研究，定量检测了癌基因Ｃ－ｍｙｃ、ｂｃｌ－２及抑癌基因ｐ５３蛋白。结果表明：经无毒剂量的千斤藤碱及维甲酸处理后，头颈部恶性肿瘤细胞株的细胞形态趋向良性分化，细胞接触抑制部分恢复，集落形成率和细胞增殖指数明显降低，癌基因Ｃ－ｍｙｃ、ｂｃｌ－２蛋白明显降低，ｐ５３蛋白明显增高。结论：千斤藤碱及维甲酸对头颈部恶性肿瘤有诱导分化作用，其机理可能是通过抑制癌基因的表达，增加抑癌基因的表达，改变细胞的形态结构和生物学特性，促进癌细胞的分化。

Differentiation inducing effect of stepholidine and retinoic acid on human head and neck carcinoma

To observe the differentiation inducing effect of Stepholidine (ST) and Retinoic Acid (RA) on laryngeal and nasopharyngeal carcinoma, the expression of oncogene (C myc, bcl 2) protein and tumor suppresser gene (p53) was done by using flow cytometry and ABC immunohistochemical methods combined with image analysis technique. The results indicated that after treated with ST and RA, the cells became well differentiated, the cell growth was suppressed, contact suppress was partially recovered, colonyforming was decreased, protooncogenes (C myc bcl 2) protein was decreased, tumor suppresser gene (p53) protein was increased. No significant difference was observed between the cells treated with ST and TA. We believed that the Chinese medicine Stephania might be expected to become a new prospective differentiation inducer in carcinoma of head and neck.