Autoradiographic and cytochemical localization of androgen in human prostatic cancer cell lines |
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| Authors: | Y Katsuoka H Hoshino M Shiramizu K Sakabe K Seiki |
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| Affiliation: | 1. Human and Environmental Toxicology, Department of Biology, University of Konstanz, Konstanz, Germany;2. Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, USA;1. Institute of Translational Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany;2. Experimental and Translational Research Center, Laboratory of Translational Medicine in Liver Cirrhosis, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China;3. Liver Research Center, Laboratory of Translational Medicine in Liver Cirrhosis, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China;4. Beijing Clinical Medicine Institute, Beijing, P.R. China;5. National Clinical Research Center of Digestive Diseases, Beijing, P.R. China;6. Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan;7. Boehringer-Ingelheim, Cardiometabolic Research, Biberach, Germany;8. Research Center for Immunotherapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany;9. Division of Gastroenterology Beth Israel Deaconess Medical Center, Harvard Medical School Boston, Boston, Massachusetts;1. Application Technology Research and Development Center of Traditional Chinese Medicine in Hebei Universities, Hebei University of Chinese Medicine, Shijiazhuang 050200, PR China;2. Hebei Technological Innovation Center of Chiral Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050026, China;3. Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong Province 250012, China;1. Department of Biodiversity and Environmental Management (Botany), Mountain Livestock Institute (CSIC-ULE), Faculty of Biological and Environmental Sciences, University of León, Campus de Vegazana s/n. E-24071, Spain;2. Faculty of Sciences and Technology, Campus de Gambelas, University of Algarve, Faro 8005-139, Portugal;3. CCMAR—Centre of Marine Sciences (CCMAR), Campus de Gambelas, University of Algarve, Faro 8005-139, Portugal;4. Department of Animal and Plant Biology and Ecology, Botanical Section, University of Jaén Spain. Campus Lagunillas s/n, Spain;5. Department of AGRARIA, “Mediterranea” University of Reggio Calabria, Reggio Calabria, Italy;6. Department of Landscape, Environment and Planning, Mediterranean Institute for Agriculture, Environment and Development (MED), The Institute for Earth Sciences—ICT, School of Science and Technology, University of Évora, Portugal;1. Department of Chemistry, University of Missouri – Kansas City, MO 64110, USA;2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China;3. College of Forestry and Landscape Architecture, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, Key Laboratory of Biomass Energy of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou, 510642, PR China |
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| Abstract: | For basic studies of receptor dynamics in androgen-responsive tissues and cells, the autoradiographic and cytochemical procedures were applied to cultured tumor cells (DU-145 and PC-3). Uptake and retention of 3H-R1881, a potent synthetic androgen, were observed in DU-145 cells. The radioactive labelling was intense, and solely confined to the nuclei of DU-145 cells. Radioactivity over PC-3 cells was minimal. For assessing binding specificity, DU-145 cells were incubated with 3H-R1881 in the presence or absence of either unlabelled R1881, testosterone, progesterone, estradiol-17 beta, or corticosterone. The displacement of 3H-R1881 with R1881 and testosterone was significant, while no displacement was observed with other steroids. Nuclear localization of cytochemical staining of the dihydrotestosterone-peroxidase conjugate was evident in DU-145 cells. Our results indicate that androgen receptor may reside primarily in target cell nuclei of androgen-responsive tissues and tumors. |
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