| 蛋白激酶C抑制剂H-7抑制肿瘤坏死因子对骨细胞的作用(英文) |
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| 引用本文： | 吴堂明,张俊平,胡振林,钱定华.蛋白激酶C抑制剂H-7抑制肿瘤坏死因子对骨细胞的作用(英文)[J].中国药理学报(英文版),1995(6). |
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| 作者姓名： | 吴堂明 张俊平 胡振林 钱定华 |
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| 作者单位： | 第二军医大学药学院中西药研究室，第二军医大学药学院中西药研究室，第二军医大学药学院中西药研究室，第二军医大学药学院中西药研究室 上海 200433 中国，上海 200433 中国，上海 200433 中国，上海 200433 中国 |
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| 基金项目： | National Natural Science Foundation of China, №39070944 |
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| 摘 要： | 用富含成骨细胞的小鼠头盖骨细胞测试肿瘤坏死因子(TNF)对骨细胞的作用以及蛋白激酶C(PK C)抑制剂H-7对TNF的影响.方法:~3H]TdR参入法观察细胞增殖.以对-硝基苯酚磷酸酯为底物用微量分光光度法测定细胞内碱性磷酸酶和耐酒石酸酸性磷酸酶活性.结果:TNF(1—100 kU·L~(-1))以剂量依赖方式抑制骨细胞增殖和碱性磷酸酶活性,但增强耐酒石酸酸性磷酸酶活性.H-7(5—20μmol·L~(-1))对TNF上述作用具有显著抑制作用且呈剂量依赖.结论:TNF对骨细胞的作用与PK C有关.
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| 关 键 词： | 肿瘤坏死因子 蛋白激酶C 成骨细胞 培养的细胞 哌嗪 H-7 |
Protein kinase C inhibitor H-7 blocks effects of tumor necrosis factor on bone cells |
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| WU Tang-Ming,ZHANG Jun-Ping,HU Zhen-Lin,QIAN Ding-Hua.Protein kinase C inhibitor H-7 blocks effects of tumor necrosis factor on bone cells[J].Acta Pharmacologica Sinica,1995(6). |
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| Authors: | WU Tang-Ming ZHANG Jun-Ping HU Zhen-Lin QIAN Ding-Hua |
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| Abstract: | To study the effects of tumor necrosis factor (TNF) on the neonatal mouse os-teoblast-enriched calvarial cells and effects of protein kinase C (PK C) inhibitor, l-(5-iso-quinolinesulfonyl )-2-methylpiperazine (H-7 ) on the TNF actions. METHODS: 3H] TdR uptake by the osteoblasts was used to measure cell proliferation. Cellular alkaline phos-phatase (AlP) and tartrate resistant acid phosphatase (trAcP) activities were determined spectrophotometrically. RESULTS: TNF (1 - 100 kU.L-1) inhibited both proliferation and expression of AlP activity, but stimulated trAcP activity. These TNF-induced actions were blocked by simultaneous addition of H-7 (5-20umol.L-1). CONCLUSION : TNF has potent effects on the osteoblasts , and the blockade of TNF actions by H-7 suggests that TNF exert its effects through PK C. |
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| Keywords: | tumor necrosis factor protein kinase C osteoblasts cultured cells piperazines H-7 |
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