肝癌相关膜蛋白抗原(HAg18-1)检测诊断原发性肝细胞癌

目的本文旨在验证肝癌相关膜蛋白抗原的灵敏度,特异性和临床应用价值.方法作者采用抗人肝癌单克隆抗体(McAb)制备的 HAg18-1血清快速酶联免疫检测试剂,检查原发性肝细胞癌(PHCC)100例,肝胆管癌(HBC)5例,转移性肝癌(MHC)10例,乙型肝炎(HB)20例,肝硬变(Cir)20例,胃肠道恶性肿瘤20例,胃肠道炎症性疾病(含溃疡)20例.同时应用甲胎蛋白(AFP)对上述病例进行联检.另用血库血20例作对照检测.结果 HAg18-1阳性率 PHCC 81%,HBC 20%,MHC 10%,HB 10%,Cir 10%,胃肠道恶性肿瘤10%,胃肠道炎症性疾病5%,血库血0%.AFP 阳性率 PHCC 68%,HBC 40%,MHC 20%,HB 20%,Cir 20%,胃肠道恶性肿瘤15%,胃肠道炎症性疾病10%,血库血0%.结论 HAg18-1对 PHCC 诊断阳性率高,特异性强,与 AFP 联检,可起协同和互补作用.

Clinical use of hepatic carcinoma associated membrane protein antigen (HAg18-1) as reagent of enzyme linked immunosorbent assay for detection of primary hepatocellular carcinoma

AIMS To evaluate the sensitivity,specificity and clinical value of hepatic carcinoma associated mem- brane protein antigen (HAg18-1) used as a reagent of serum quick enzyme linked immunosorbent assay (ELISA) for the detection of primary hepatocellular car- cinoma (PHCC). METHODS Serum quick enzyme linked immunosor- bent assay (ELISA) with HAg18-1 as reagent,which was prepared by monoclonal antibody against human hepatic carcinoma,was performed on 100 cases of pri- mary hepatocellular carcinoma (PHCC),5 cases of hepatic biliary carcinoma (HBC),10 cases of metastatic hepatic carcinoma (MHC),20 cases of hep- atitis B (HB),20 cases of liver cirrhosis (LC),20 cas- es of gastrointestinal malignant tumours and 20 cases of gastronintestinal inflammatory diseases (including ulcers). Alpha-fetoprotein (AFP) detection concurrent- ly for each case. Twenty samples of bank blood were tested as controls. RESULTS Positive rate of HAg18-1 ELISA and AFP detection were 81% and 68% in PHCC,20% and 40% in HBC,19% and 20% in MHC,10% and 20% in BH, 10% and 20% in LC,10% and 15% in gastrointestinal malignant tumors,and 5% and 10% in gastrointestinal inflammatory diseases. No positive result of HAg18-1 ELISA and AFP detection occurred in any sample of bank blood. CONCLUSIONS HAg18-1 ELISA has high sensitivity and specificity in the detection of PHCC. HAg18-1 ELISA and AFP detection,if used together,may be complementary to each other in the diagnosis of PHCC.