| 大鼠肾小球系膜细胞活性氧簇JAK2/STAT5通路与TIMP-1之间的关系及对其凋亡的影响 |
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| 引用本文： | 温文斌,林洪丽,吴泰华,马艳梅,夏丽华,单路娟.大鼠肾小球系膜细胞活性氧簇JAK2/STAT5通路与TIMP-1之间的关系及对其凋亡的影响[J].长治医学院学报,2010,24(5). |
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| 作者姓名： | 温文斌 林洪丽 吴泰华 马艳梅 夏丽华 单路娟 |
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| 作者单位： | 长治医学院附属和济医院肾内科,046011;大连医科大学附属第一医院;长治医学院附属和济医院消化内科;内蒙古医学院附属医院 |
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| 摘 要： | 目的:探讨高糖(HG)条件下大鼠肾小球系膜细胞(RMC)活性氧簇(ROS)、JAK2/STAT5信号通路与金属蛋白酶1组织抑制剂(TI MP-1)之间的关系及对其凋亡的影响。方法:在HG培养条件下的RMC中,根据是否使用DPI(NADPH氧化酶特异性抑制剂,可抑制ROS产生)和AG490(JAK2特异性抑制剂)刺激24 h,将RMC分为HG组、HG+AG490组、HG+DPI组和HG+AG490+DPI组四组。采用流式细胞技术检测各组细胞的凋亡率,RT-PCR检测各组RMC Bcl-xl、Cyclin D1、P27kip1、JAK2和TI MP-1 mRNAs的表达,Western blot检测胞浆中JAK2、STAT5及相应磷酸化蛋白的表达。结果:HG组、HG+AG490组、HG+DPI组和HG+AG490+DPI组的细胞总凋亡率分别为:(10.69±0.26)%、(20.52±0.51)%、(24.56±0.36)%和(26.01±0.28)%;加入AG490和(或)DPI后RMC总凋亡率明显升高(P<0.01),Bcl-xl、Cyclin D1、JAK2和TI MP-1 mRNAs表达显著减少,P27k...
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| 关 键 词： | 系膜细胞 活性氧簇 JAK2/STAT5信号通路 金属蛋白酶1组织抑制剂 凋亡 |
The Relationship Among ROS,JAK2/STAT5 Signal Pathway and TIMP-1 and the Effect of them on Apoptosis in Rat Mesangial Cells |
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| Wen Wenbin,Lin Hongli,Wu Taihua,et al..The Relationship Among ROS,JAK2/STAT5 Signal Pathway and TIMP-1 and the Effect of them on Apoptosis in Rat Mesangial Cells[J].Journal of Changzhi Medical College,2010,24(5). |
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| Authors: | Wen Wenbin Lin Hongli Wu Taihua |
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| Institution: | Wen Wenbin,Lin Hongli,Wu Taihua,et al.Department of Nephrology,Heji Hospital,Changzhi Medical College |
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| Abstract: | Objective:To investigate the relationship among Reactive Oxygen Species(ROS),JAK2/STAT5 signal pathway and TIMP-1 and the effect of them on apoptosis in rat mesangial cells under the condition of high glucose.Methods:After RMC was stimulated with HG,DPI(specific inhibitor of NADPH oxidase) and AG490(specific inhibitor of JAK2) for 24 hours,the total apoptosis rate of RMC was measured by flow cytometry.The expression of Bcl-xl,Cyclin D1,P27kip1,JAK2 and TIMP-1 mRNAs was assayed by RT-PCR.The expression of JA... |
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| Keywords: | Mesangial cells Reactive Oxygen Species JAK2/STAT5 signal pathway Tissue inhibitor of melalloproteinase 1 Apoptosis |
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