不同产地菟丝子总黄酮及金丝桃苷的含量测定 |
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引用本文: | 管群,吕圭源,俞静静,颜美秋,陈素红.不同产地菟丝子总黄酮及金丝桃苷的含量测定[J].浙江中医药大学学报,2012,36(2):184-186,191. |
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作者姓名: | 管群 吕圭源 俞静静 颜美秋 陈素红 |
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作者单位: | 1. 温州医学院浙江,温州,325035 2. 浙江中医药大学 |
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基金项目: | 国家重点基础研究发展计划(973计划)(2007CB512603)~~ |
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摘 要: | 目的]用紫外可见分光光度法测定不同产地(宁夏、内蒙古、辽宁、江苏、河北、陕西、四川、山东)菟丝子中总黄酮的含量;用高效液相色谱(HPLC)法测定金丝桃苷的含量。方法]紫外可见分光光度法采用NaNO2-Al(NO3)3-NaOH体系显色,以芦丁为对照,在504nm波长处测定吸光度;HPLC法采用Ultimate XB-C18色谱柱(250mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸溶液(17:83),流速:1.0 mL.min-1,柱温:30℃,检测波长:360nm。结果]不同产地菟丝子药材中的总黄酮含量最高为2.55%,最低为0.38%;金丝桃苷含量最高为0.276%,最低为0.012%。结论]不同产地菟丝子药材中总黄酮及金丝桃苷含量均存在较大差异,且总黄酮含量越高,金丝桃苷含量相应也高,二者呈一定相关性。
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关 键 词: | 菟丝子 产地 总黄酮 金丝桃苷 含量测定 |
Quantitative Determination of Total Flavones and Hyperoside in Cuscuta Chinensis Lam Collected from Different Places |
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Institution: | Guan Qun,Lv Guiyuan,Yu Jingjing,et al .Wenzhou Medical College,Zhejiang(325035);2.Zhejiang Chinese Medical University |
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Abstract: | Objective] Ultraviolet-Visible spectrophotometer(UV) and HPLC methods were developed to determine the content of total flavones and hyperoside in Cuscuta chinensis Lam collected from different places(Ningxia,Neimenggu,Liaoning,Jiangsu,Hebei,Shanxi,Sichuan and Shandong). Methods] Rutin was used as the standard to measure the total flavones,the colour reactoin was carried out with NaNO2-Al(NO3)3-NaOH and measu-red in the 504 nm wavelength by UV spectrophotometry.The hyperoside was separated with acetonitrile-0.1%H3PO4(17:83)(flow rate set at 1.0 mL·min-1,wavelength was 360 nm) by Ultimate XB-C18(250 mm×4.6 mm,5 μm) column.Results] The total flavones contained in Cuscuta chinensis was from 0.38% to 2.55%;the amounts of hyperoside was from 0.012% to 0.276%.Conclusion] The amounts of both total flavones and hyperoside were of significantly different Cuscuta chinensis Lam from different places,and there was a significant correlation between the contents of total flavones and the content of the hyperoside in the same sample: namely the higher the level of the total flavones,the higher the content of hyperoside. |
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Keywords: | Cuscuta chinensis Lam different places total flavones hyperoside content determination |
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