汉族急性脑血管病患者血浆血栓调节蛋白的变化和血栓调节蛋白基因Ala455Val(C1418T)多态性研究

目的 探讨急性脑血管病患者血浆可溶性血栓调节蛋白(soluble thrombomodulin,sTM)含量的变化和血栓调节蛋白基因Ala455Val(C1418T)多态性的分布.方法 在79例汉族急性脑血管病患者(脑出血患者30例,脑梗死患者49例)和30名年龄和性别相匹配的健康对照者中,采用聚合酶链反应限制性酶切片段长度多态性分析技术检测TM Ala455Val(C1418T)多态性,酶联免疫吸附试验检测血浆sTM含量.结果 脑梗死组和脑出血组sIM含量分别为(32.08±6.98)ng/ml和(38.93±6.77)ng/ml,显著高于正常对照组的(9.90±3.09)ng/ml(P均<0.01),而且与高血压、糖尿病、心脏病等高危因素无关;脑出血组和脑梗死组TM 1418C/C基因型频率分别为53.3%和57.1%,高于对照组的40%,但无统计学差异.结论 无论足脑出血还是腑梗死患者,血浆sTM含量均显著高于正常人群;汉族人群中脑出血和脑梗死患者TM 1418C/C基因型频率虽然高于正常人群,但并非脑血管病的易感基因.

Changes of thrombomodulin and thrombomodulin Ala455Val polymorphism (C14181) in Chinese Han patients with acute cerebrovascular diseases

Objective To investigate the changes of plasma soluble thrombomodulin (sTM) and the distribution of thrombomodulin Ala455Val (C1418T) polymorphism in patients with acute cerebrovascular diseases. Methods Among the 79 Chinese Han patients with acute cerebrovascular disease (30 intracerebral hemorrhage, and 49 cerebral infarction) and 30 age-and sex-matched healthy controls, polymerase chain reaction-restriction fragment length polymor phism (PCR-RFLP) analysis was used to detect TM Ala455Val (C1418T) polymorphism, and enzyme-linked immunosorbent assay (ELISA) was used to detect sTM level. Results The sTM levels in the cerebral infarction and intracerebral hemorrhage groups were 32. 08 ± 6. 98 ng/ml and 38. 93 ±6. 77 ng/ml, respectively. They were significantly higher than 9. 90 ±3. 09 ng/mL in the healthy control group (all P < 0. 01) and were not associated with the high risk factors, such as hypertension, diabetes, and cardiac disease etc; TM 1418C/C genotype frequencies in the intthe intracerebral hemorrhage and cerebral infarction groups were 53. 3% and 57.1% respectively, and they were higher than 40% of the control group, but there was no statistically significant differences. Conclusions No matter the patients with intracerebral hemorrhage or cerebral infarction, the plasma sTM levels were significantly higher than the normal population; although TM 1418C/C genotype frequencies in patients with intracerebral hemorrhage and cerebral infarction in Chinese Han population were higher than normal population, they were not the susceptible genes of cerebrovascular disease.