| 应用基因芯片技术筛选转染NOR1基因后HepG2细胞差异表达基因 |
| |
| 引用本文： | 申春梅,聂新民,李登清. 应用基因芯片技术筛选转染NOR1基因后HepG2细胞差异表达基因[J]. 实用预防医学, 2007, 14(3): 609-611 |
| |
| 作者姓名： | 申春梅 聂新民 李登清 |
| |
| 作者单位： | 1. 中南大学湘雅医学院医学检验系,湖南,长沙,410013
2. 中南大学湘雅三医院检验科 |
| |
| 基金项目： | 国家自然科学基金项目(基金编号:30300383) |
| |
| 摘 要： | 目的研究转染NOR1基因后肝癌细胞系HepG2基因表达谱的改变。方法应用脂质体介导的转染方法将pcDNA3.1( )/NOR1真核表达载体和pcDNA3.1( )空白载体分别转染入肝癌细胞系HepG2,再分别提取转染pcD-NA3.1( )/NOR1和空载体pcDNA3.1( )的HepG2细胞总RNA,应用基因芯片技术进行芯片分析。结果芯片分析结果显示差异表达基因中上调的基因有59个,下调的基因有103个,这些差异基因的功能涉及多个方面。结论应用基因芯片技术成功筛选了NOR1基因转染细胞后差异表达基因,为阐明NOR1基因与肝癌发生发展的关系提供了实验依据。
|
| 关 键 词： | NOR1基因 基因芯片 HepG2细胞 |
| 文章编号： | 1006-3110（2007）03-0609-03 |
| 收稿时间： | 2007-04-06 |
| 修稿时间： | 2007-04-06 |
Screening of Genes Differentially Expressed in HepG2 Cells Transfected With NOR1 Gene Using DNA Microarray |
| |
| SHEN Chun-mei,NIE Xin-min,LI Deng-qing. Screening of Genes Differentially Expressed in HepG2 Cells Transfected With NOR1 Gene Using DNA Microarray[J]. Practical Preventive Medicine, 2007, 14(3): 609-611 |
| |
| Authors: | SHEN Chun-mei NIE Xin-min LI Deng-qing |
| |
| Affiliation: | Department of Medical Laboratory, Xiangya Medical School of Central South University, Changsha 410013, Hunan, China |
| |
| Abstract: | Objective To investigate the effects of NOR1 gene on the expression profile of hepatoma cell line HepG2.Methods The HepG2 cells were transfected by pcDNA3.1( )and pcDNA 3.1( )-NOR1 by using the lipofectamin transfection reagent respectively.The RNA was isolated.High-throughout DNA microarray was used to screen the effects of NOR1 on the expression profile of hepatoma cell line HepG2.Results The DNA microarray data suggested that changes of the expression profile induced by NOR1 transfection were very wide.The up-regulated and down-regulated genes were amount to 59 and 103 respectively.The most important altered genes belonged to many functional categories.Conclusion The influence of NOR1 on the expression profile of hepatoma cell line HepG2 is extensive.The altered genes may be useful to clarify the mechanism of hepatocarcinogenesis. |
| |
| Keywords: | NOR1 DNA microarray HepG2 cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
