Biochemical metabolism and oxygen free radical changes following ischemic and reperfused injured limbs. An experimental study

Oxygen free radicals may contribute to skeletal muscle damage associated with ischemia and reperfusion. This study utilized rabbit right hindlimb ischemic model to investigate the biochemical metabolism and oxygen free radicals change in limbs subjected to periods of ischemia and reperfusion. The results showed that the levels of malondialdehyde (MDA) as the oxygen free radical product in the plasma from ischemic and reperfused limb was significantly higher than that from the single ischemic limb, and varied with intracellular enzymes (CPK, LDH, GOT) which reflected the injury of skeletal muscle cells after ischemia. It Suggests that increased free radical product might be one of the important causes of ischemic and reperfused injury in limbs.