| 旋毛虫抗原基因Ts87在真核细胞中的表达 |
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| 引用本文： | 徐旭娜,诸欣平,杨雅平,杨静,张路平. 旋毛虫抗原基因Ts87在真核细胞中的表达[J]. 中国病原生物学杂志, 2003, 16(2): 71-73 |
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| 作者姓名： | 徐旭娜 诸欣平 杨雅平 杨静 张路平 |
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| 作者单位： | 1. 河北师范大学生命科学学院,河北,石家庄,050016
2. 首都医科大学基础医学院寄生虫学教研室,北京,100054 |
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| 基金项目： | 美国中华医学会 (CMB) (Parasitology 98- 674)，国家留学基金回国科研资助 (1 998～ 2 0 0 1 )，北京市教委科技发展基金 (0 1 KJ- 0 91 )，北京市跨世纪优秀人材工程 (2 0 0 1～ 2 0 0 3)，河北省自然科学基金 (30 2 1 4 3)资助项目 |
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| 摘 要： | 目的 在体外真核细胞 COS7中表达重组质粒 pc DNA3.1/ Ts87,为旋毛虫病 DNA疫苗的研究奠定基础。 方法 将重组质粒 pc DNA3.1/ Ts87经脂质体 L ipofectamine介导 ,体外转染真核细胞 COS7,通过细胞免疫组化 ,细胞裂解物的 SDS- PAGE电泳和 Western- blot分析检测表达产物。 结果 重组质粒能够在 COS7中表达出约 38ku的目的蛋白 ,并能够被旋毛虫阳性血清特异识别。 结论 构建的重组质粒可在体外真核细胞 COS7中成功表达。
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| 关 键 词： | 旋毛虫 COS7细胞 重组质粒 真核表达 |
| 文章编号： | 1001-6627(2003)02-0071-03 |
| 修稿时间： | 2002-07-04 |
EXPRESSION IN COS7 CELL LINE OF Ts87 GENE ENCODING ANTIGEN FROM TRICHINELLA SPIRALIS |
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| XU Xu na ,ZHU Xin ping ,YANG Ya ping ,YANG Jing ,ZHANG Lu ping. EXPRESSION IN COS7 CELL LINE OF Ts87 GENE ENCODING ANTIGEN FROM TRICHINELLA SPIRALIS[J]. Journal of Pathogen Biology, 2003, 16(2): 71-73 |
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| Authors: | XU Xu na ZHU Xin ping YANG Ya ping YANG Jing ZHANG Lu ping |
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| Affiliation: | XU Xu na 2,ZHU Xin ping 1,YANG Ya ping 1,YANG Jing 1,ZHANG Lu ping 2 |
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| Abstract: | Objective To express recombinant plasmid pcDNA3.1/Ts87 in COS7 cells in vitro , so as to lay a foundation for developing DNA vaccine against Trichinellosis. Methods The pcDNA3.1/Ts87 was transfected into COS7 cells by lipofectamine reagent, and its expression product was identified by immunohistochemical assay, SDS PAGE and Western blot. Results The results of SDS PAGE and Western blot revealed that the molecular weight of the expressed protein was approximately 38 ku and the expressed protein can be specifically recognized by sera from rabbit infected by Trichinella spiralis , sera from rabbit immunized with soluble antigen of T. spiralis , sera from rabbit immunized with Ts87 recombinant protein and a monoclonal antibody of T. spiralis , respectively. Conclusion The Ts87 gene was successfully expressed in the COS7 cells. |
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| Keywords: | Trichinella spiralis COS7 cells recombinant plasmid eukaryotic expression |
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