p21WAF1、Cyclin D1和PRb在膀胱移行细胞癌中的表达及其意义

目的 :探讨 p2 1WAF1、细胞周期蛋白 D1 (Cyclin D1 )和 PRb在膀胱移行细胞癌(BTCC)中的表达及相互关系及其意义。方法 :应用免疫组织化学 SP法检测 5 7例 BTCC患者癌组织中 p2 1WAF1、Cyclin D1 和 PRb的蛋白表达。结果 :p2 1WAF1、Cyclin D1 和 PRb的阳性表达率分别为36 .8%、49.1%和 45 .6 % ,p2 1WAF1随病理分级升高阳性率显著下降 ,Cyclin D1 和 PRb的表达与BTCC的病理分级、临床分期和有无转移均相关 ,p2 1WAF1与 PRb的表达呈负相关 ,Cyclin D1 和PRb的表达呈正相关 ,而 p2 1WAF1与 Cyclin D1 的表达无关。结论 :p2 1WAF1/ Cyclin D1 / PRb通路异常与 BTCC的发生发展密切相关 ,p2 1WAF1的改变可能为癌变的早期事件 ,联合检测 p2 1WAF1、Cyclin D1 和 PRb可较准确地评价 BTCC的生物学特性 ,估计预后 ,指导治疗

Expression of p21WAF1, Cyclin D1 and PRb in Bladder Transitional Cell Carcinoma

Objective:To investigate the expression and their significance of p21WAF1,cyclin D 1 and PRb in human bladder transitional cell carcinoma (BTCC),and the relationships among them.Methods:Immunohistochemistry was applied to detect the expression of p21WAF1,cyclin D 1 and PRb in 57 cases of BTCC.Results:Positive rates of p21WAF1,cyclin D 1 and PRb in 57 cases of BTCC were 36.8 %,49.1 % and 45.6 %,respectively.p21WAF1 gene expression was related to the tumor grade (P<0.005).Cyclin D 1 and PRb gene expressions were related to the tumor grade,clinic stage and metastasis (P<0.05).p21WAF1 gene expression was negtively ralated to PRb gene expression (P<0.005,Kappa=-0.401),and cyclin D 1 gene expression was positively ralated to PRb gene expression (P<0.01,Kappa=0.367),while p21WAF1 gene expression was not related to cyclin D 1 gene expression (P>0.05).Conclusion:Alteration in at least one of the three genes might be required for most BTCC occurence and develepment.Loss of the p21WAF1 protein may be an early event in tumorigenesis.The analysis of multiple genes are more valuable in evaluating the biological character of BTCC.