3H] (-)sulpiride binding in rat striatum, cortex and anterior pituitary: an improved assay |
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| Authors: | E Stefanini A M Ortu F Vernaleone G L Gessa |
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| Affiliation: | Department of Neuroscience, School of Medicine, University of Cagliari, Italy. |
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| Abstract: | The interaction of [3H] (-)sulpiride with D2 dopamine (DA) receptors in the striatum, anterior pituitary and medial-prefrontal cortex was studied in rats, using an improved [3H] (-)sulpiride radioreceptor binding technique. Incubation on ice and a fast filtration resulted in higher specific binding (approximately 85% of total binding) a lower affinity constant (about 3 nM) and higher Bmax than reported with previous procedures. Pharmacological interactions confirmed the high selectivity of [3H] (-)sulpiride for D2 DA receptors. |
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