应用荧光原位杂交技术检测肺癌患者染色体数目的改变

目的应用荧光原位杂交(FISH)技术研究肺癌患者染色体数目改变及其意义。方法用人的全套染色体特异探针与肺癌患者中期染色体进行杂交检测。结果肺癌患者细胞为异倍体,染色体数目以亚二倍体居多,常见2、5、7、8、10、11、14和17号染色体增多及1、4号染色体的丢失。结论 FISH技术可检测肺癌患者染色体数目的改变,肺癌遗传学变异主要是2、5、7、8、10、11、14、17号染色体的增加,并对肺癌的发生、发展和预后有一定的提示作用。

Detection of chromosome number abnormalities in lung cancer by fluorescence in situ hybridization.

Objective： To study the chromosome number abnormality in lung cancer by fluorescence in situ hybridization （FISH）. Methods： The metaphases of lung cancer specimen were hybridizated by FISH with the whole set of human chromosome - specific probes （ HSA1 -22, X, plus Y）. Results： The lung cancer cells were heteropioid, the chromosome number was predominately hypodiploid. FISH analysis demonstrated an increase of chromosome 2, 5, 7, 8, 10, 21, 14, and 17, and loss of chromosome 1 and chromosome 4 appeared frequently. Conclusion ： FISH can detect the chromosome number changes of lung cancer. The genetic mutation of lung cancer is marly characterized by increased number of chromosome 2, 5, 7, 8, 20, 12, 14, and 17, which will provide some clue for development and prognosis of lung cancer.