| HPLC法同时测定南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量 |
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| 引用本文： | 马梅芳,吕文海.HPLC法同时测定南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量[J].中国药事,2009,23(1):65-67. |
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| 作者姓名： | 马梅芳 吕文海 |
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| 作者单位： | 山东中医药大学,济南,250014 |
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| 摘 要： | 目的 建立南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量测定方法。方法 采用Diamond—C18色谱柱；甲醇-0．4％磷酸溶液（50：50）为流动相；流速1．0mL·min；检测波长360nm。结果 线性范围：槲皮素0．1184～0．8288μg，r=0．9995，山奈酚0．0258～0．1806／μg，r=0．9994，异鼠李素0．0444～0．3108μg，r=0．9994；平均回收率：槲皮素99．51％，RSD=2．10％（n=6），山奈酚101．03％，RSD=2．08%（n=6），异鼠李素99．80％，RSD=2．09％（n=6）。结论 该法操作简便、准确，重复性好，可用于南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量测定。
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| 关 键 词： | 南葶苈子 炮制 槲皮素 山奈酚 异鼠李素 HPLC 含量测定 |
Simultaneous Determination of Quercetin,Kaempferol and Isorhamnetin in Descurainia Sophia (L.) Webb ex Prantl Processed by HPLC |
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| Ma Meifang,Lv Wenhai.Simultaneous Determination of Quercetin,Kaempferol and Isorhamnetin in Descurainia Sophia (L.) Webb ex Prantl Processed by HPLC[J].Chinese Pharmaceutical Affairs,2009,23(1):65-67. |
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| Authors: | Ma Meifang Lv Wenhai |
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| Institution: | (Shandong University of TCM, Jinan 250014) |
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| Abstract: | Objective The Method of determine the quercetin, kaempferol and isorhamnetin in De- scurainia Sophia （L.） Webb ex Prantl processed by HPLC was established. Methods The column was Di- amond-C18 Column, the mobile phase was methanol-0.4% phosphoric acid （50：50）, the flow rate was 1.0mL·min^-1 and the detection wavelength was 360nm. Results The linear range of quercetin was 0. 1184-0.8288μg （r=0.9995） , the average recovery was 99.51%, RSD=2.10% （n=6）. The linear range of kaempferol was 0. 0258-0. 1806μg （r=0. 9994） , the averag recovery was 101.03%, RSD=2.08%（n=6）. The linear range of isorhamnetin was 0. 0444-0. 3108μg （r=0. 9994） , the average recovery was 99.80%, RSD=2.09%（n=6）. Conclusions This method is simple, rapid and accurate with good reproducibility, it is suitable for the quality control of Descurainia Sophia （L.） Webb ex Prantl processed. |
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| Keywords: | HPLC |
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