肺气肿模型大鼠肾组织水通道蛋白2的表达及机制研究

目的: 观察肺气肿模型大鼠肾组织AQP2表达以及血中ET、IL-1β、TNF-α水平的变化,探讨在体液代谢过程中肺与肾脏之间关系。方法: 用气管内注入脂多糖加熏香烟方法复制肺气肿大鼠模型,应用免疫组化、Western blotting、RT-PCR方法测定肾组织AQP2蛋白及mRNA表达的变化,应用放免法测定血中、肺组织匀浆中ET、IL-1β及TNF-α含量。结果: 与对照组比较,肾组织AQP2蛋白及mRNA表达增强(P<0.01);同时模型组血、肺组织ET、IL-1β及TNF-α含量明显升高(P<0.01)。结论: 肺气肿模型大鼠肾组织AQP2表达上调;其机制可能与血中ET、IL-1β、TNF-α含量升高有关。

Alteration of AQP2 expression in kidney tissues of emphysema model rats

AIM: To observe the expressions of aquaporin 2 (AQP2) in kidney tissues and the contents of endotoxin (ET), interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α) in serum in emphysema model rats, and to investigate the relationship between lungs and kidney in humoral metabolism. METHODS: The rats of emphysema were treated by injecting lipopolysaccharide into the trachea with cigarette smoking. Immunohistochemistry and Western blotting analysis were used to observe the expression of AQP2 in kidney tissues. RT-PCR was applied to detect the expression of AQP2 mRNA in kidney tissues. Blood sample and lung tissue were taken and the levels of ET, IL-1β and TNF-α were measured by radioimmunoassay. RESULTS: AQP2 expression in the kidney tissue in model group was greater than that in control group, and the expression of AQP2 mRNA showed the same results (P<0.01). ET, IL-1β and TNF-α levels in serum and lung tissue in model group were markedly higher than those in control group (P<0.01). CONCLUSION: In the emphysema model rats, AQP2 expression is up-regulated in the kidney tissue. The mechanism of emphysema may be related to increasing the levels of ET, IL-1β and TNF-α in the serum and lung tissue obviously.