| 3,6-二羟基黄酮对多种肿瘤细胞的增殖抑制及凋亡诱导作用 |
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| 引用本文： | 常徽,糜漫天,顾艳艳,袁家林,凌文华,林辉. 3,6-二羟基黄酮对多种肿瘤细胞的增殖抑制及凋亡诱导作用[J]. 第三军医大学学报, 2009, 31(16): 1566-1568 |
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| 作者姓名： | 常徽 糜漫天 顾艳艳 袁家林 凌文华 林辉 |
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| 作者单位： | 第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室,重庆,400038;中山大学公共卫生学院营养系,广州,510080 |
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| 摘 要： | 目的 观察3,6-二羟基黄酮对乳腺癌细胞株MCF-7(雌激素受体阳性ER+)、MDA-MB-231(雌激素受体阴性ER-)、直肠癌细胞株LoVo、DLD-1和前列腺癌细胞株PC3增殖和凋亡的影响.方法 MTT法检测3,6-二羟基黄酮对不同肿瘤细胞生长存活的影响,计算半数抑制浓度(IC50);DAPI染色观察3,6-二羟基黄酮对肿瘤细胞生长状态及形态的影响,Annexin V/PI双染流式细胞仪检测细胞凋亡变化.结果 增殖检测结果表明,3,6-二羟基黄酮对于MCF-7、MDA-MB-231、LoVo、DLD-1和PC3细胞增殖均表现出显著抑制作用,48 h IC50值分别为16.1、15.7、29.8、12.8和17.8μmol/L;细胞形态观察和凋亡检测结果表明,10 μmol/L的3,6-二羟基黄酮作用24 h,5种肿瘤细胞的生长状态和形态均发生显著变化,大量细胞表现出凋亡或死亡细胞的形态特征,细胞凋亡率均显著升高.结论 3,6-二羟基黄酮具有显著的抗肿瘤效应,对于多种肿瘤细胞具有很强的增殖抑制和凋亡诱导作用.
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| 关 键 词： | 3 6-二羟基黄酮 肿瘤细胞 增殖 凋亡 |
Antiproliferative and proapoptotic effects of 3,6-dihydroxyflavone on different cancer cells |
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| CHANG Hui,MI Man-tian,GU Yan-yan,YUAN Jia-lin,LING Wen-hua,LIN Hui. Antiproliferative and proapoptotic effects of 3,6-dihydroxyflavone on different cancer cells[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(16): 1566-1568 |
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| Authors: | CHANG Hui MI Man-tian GU Yan-yan YUAN Jia-lin LING Wen-hua LIN Hui |
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| Affiliation: | CHANG Hui1,MI Man-tian1,GU Yan-yan1,YUAN Jia-lin1,LING Wen-hua2,LIN Hui1 (1Department of Nutrition and Food Hygiene,Chongqing Key Laboratory of Nutrition and Food Safety,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038,2Department of Nutrition,Public Health School,Sun Yet-sen University,Guangzhou 510080,China) |
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| Abstract: | Objective To observe the effects of 3,6-dihydroxyflavone on the proliferation and apoptosis in breast cancer cell lines MCF-7 (ER+) and MDA-MB-231 (ER-),colorectal carcinoma cell lines LoVo and DLD-1,prostatic cancer cell line PC3. Methods Cell proliferation was detected by MTT assay. The morphological changes in cancer cells were observed after DAPI staining. Cell apoptosis was determined by flow cytometry after Annexin V/PI staining. Results MTT assay showed that 3,6-dihydroxyflavone could significantly i... |
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| Keywords: | 3,6-dihydroxyflavone cancer cells proliferation apoptosis |
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