Cone signals in monostratified and bistratified amacrine cells of adult zebrafish retina |
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Authors: | MM Torvund TS Ma VP Connaughton F Ono RF Nelson |
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Institution: | 1. National Institute of Neurological Disorders and Stroke, National Institutes of Health, Rockville Maryland;2. Graduate Program in Neuroscience, University of Arizona, Tucson, Arizona;3. University of Pennsylvania, Department of Neurosurgery, Philadelphia, PA;4. Biology Department, American University, Washington, DC;5. National Institute of Alcoholism and Alcohol Abuse, National Institutes of Health, Rockville, Maryland;6. Department of Physiology, Osaka Medical College, Takatsuki, Japan |
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Abstract: | Strata within the inner plexiform layer (IPL) of vertebrate retinas are suspected to be distinct signaling regions. Functions performed within adult zebrafish IPL strata were examined through microelectrode recording and staining of stratified amacrine types. The stimulus protocol and analysis discriminated the pattern of input from red, green, blue, and UV cones as well as the light‐response waveforms in this tetrachromatic species. A total of 36 cells were analyzed. Transient depolarizing waveforms at ON and OFF originated with bistratified amacrine types, whose dendritic planes branched either in IPL sublaminas a & b, or only within sublamina a. Monophasic‐sustained depolarizing waveforms originated with types monostratified in IPL s4 (sublamina b). OFF responses hyperpolarized at onset, depolarized at offset, and in some cases depolarized during mid‐stimulus. These signals originated with types monostratified in s1 or s2 (sublamina a). Bistratified amacrines received depolarizing signals only from red cones, at both ON and OFF, while s4 stratified ON cells combined red and green cone signals. The s1/s2 stratified OFF cells utilized hyperpolarizing signals from red, red and green, or red and blue cones at ON, but only depolarizing red cone signals at OFF. ON and OFF depolarizing transients from red cones appear widely distributed within IPL strata. “C‐type” physiologies, depolarized by some wavelengths, hyperpolarized by others, in biphasic or triphasic spectral patterns, originated with amacrine cells monostratified in s5. Collectively, cells in this stratum processed signals from all cone types. J. Comp. Neurol. 525:1532–1557, 2017. © 2016 Wiley Periodicals, Inc. |
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Keywords: | wavelength 3D‐reconstruction spectral model inner plexiform layer GE4a transgenic enhancer trap ON– OFF response ON response OFF response RRID: SCR_003070 RRID: SCR_011323 RRID: SCR_001775 RRID: SCR_002368 RRID: SCR_008593 RRID: SCR_008597 RRID: SCR_008406 RRID: SCR_013672 RRID: SCR_008960 RRID: SCR_013318 RRID: SCR_002798 |
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