人骨肉瘤鸡胚移植瘤模型的生物学特性研究

目的:建立人骨肉瘤鸡胚移植瘤模型,研究其形态学及生物学特性。方法:将人骨肉瘤细胞接种于鸡胚绒毛尿囊膜(chick embryo chorioallantoic membrane,CAM),观察影响骨肉瘤鸡胚移植瘤成活的因素、移植瘤生长特性、形态学特征和生物学性状。结果:建立了人骨肉瘤鸡胚移植瘤模型。移植瘤易于生长,具有较强的血管诱导作用。光镜下移植瘤具有与人骨肉瘤类似的组织结构。结论:该模型易于复制,能动态观察骨肉瘤诱导的血管生成过程,可用于骨肉瘤的生物学行为研究、药物筛选等领域。

Establishment of a human osteosarcoma model on the chick embryo chorioallantoic membrane and its biological properties

Objective:To establish a human osteosarcoma model on the chick embryo chorioallantoic membrane(CAM)and study its morphological and biological properties.Methods:Human osteosarcoma cell line HOS-8603 was cultivated and implanted on the CAMs.The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied.Results:A human osteosarcoma model on CAM was established.The implanted tumors were easy to grow on the CAMs and had a strong trend of angiogenesis and similar structure as human osteosarcoma with microscopic verification.Conclusion:This model is of easy duplication and simple operation,and can be used in the research of the biological properties and drug selection for human osteosarcoma.