Is ribosomal cistron activity increased in human lymphoblastoid cell lines? |
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| Authors: | N J Gupta V L Hopwood S Pathak |
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| Affiliation: | Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030. |
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| Abstract: | The genomic activity of nucleolar organizer regions (NORs) in eight human B-cell lymphoblastoid cell lines was studied following the routinely used Ag-NOR technique. The results demonstrate that (a) Ag-NORs are located in the short arms of D- and G-group chromosomes, (b) two out of eight cell lines have 66.7% and 49.0% of metaphases, respectively, with 9 to 10 active Ag-NORs, and (c) as a whole, Ag-NOR activity is much higher in B-cell lines as compared with conventional 72-hr peripheral blood lymphocyte (T-cell) harvests. |
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