合并组织学炎症的前列腺增生组织中IL-6、TNF-α表达的研究

目的:通过检测TNF-α、IL-6在单纯BPH与合并组织学炎症的BPH组织中表达的差异及两组BPH患者在临床指标上的异同,探讨炎症与BPH的发生发展的关系。方法:收集TURP术的90份BPH患者标本,用HE染色法将90份标本染色后分单纯组(A组)35份与合并炎症组(B组)55份。采用免疫组织化学方法检测TNF-α、IL-6在各标本中的表达情况,记录90例患者临床指标PSA、体积、年龄、尿流率等数据结果。全部患者均病理检查回报为BPH。结果:B组55例均为慢性炎症,构成比为0.61。与A组相比,B组患者前列腺体积较大,PSA水平也较高,差异有统计学意义(P0.05);而两组患者在年龄和尿流率比较上无统计学意义(P!0.05)。与A组比较,B组TNF-α与IL-6的表达显著增高(P0.05)。IL-6和TNF-α表达结果相关性分析:前列腺体积与患者年龄有相关性,差异有统计学意义(r=0.430,P0.001),前列腺体积与炎症程度也有相关性,差异有统计学意义(r=0.610和r=0.609,P0.001);患者的PSA水平与炎症程度有相关性,差异有统计学意义(r=0.572r=0.487,P0.01),而患者PSA水平与年龄无相关性,差异无统计学意义(r=0.065,P!0.1);通过偏倚相关分析控制年龄因素的影响后,炎症严重水平与BPH体积仍有明显相关性。结论:大部分BPH患者合并有前列腺炎症,并且以慢性炎症为主。合并炎症的BPH患者PSA水平相对较高,前列腺体积相对较大,但是在尿流率和年龄上的差异无统计学意义。IL-6、TNF-α两种促炎性因子可能对BPH和PSA的分泌起着促进作用。

Expression of IL-6 and TNF-a in BPH combined with histological inflammation

Objective： To detect the expression of TNF-α and IL-6 in simple BPH and BPH combined with prostatitis, and to evaluate the effect of inflammation on the development and progression of BPH. Method：Speci- mens of 90 patients with BPH underwent TURP were obtained. All specimens were divided histologically by HE staining into group of 35 cases of simple BPH （A group） and group of 55 cases of BPH combined with prostatitis （B group）. Protein levels expression of TNF-α and IL-6 were examined by immunohistochemistry. All patients clinical indicators including PSA, prostate volume, age, urinary flow rate were recorded, and all patients were diagnosed as BPH by pathology. Result：All patients of B group were found combined with chronic prostatitis. The constituent ratio was 61%. Patients prostate volume and PSA of B group were significantly higher than A group （P〈0.05）, but there were no significant differences in urine flow rate and patients age （P〉0.05）. As compared with A group, the expression of TNF-α and IL-6 significantly increased in B group （P〈0.05）. The results of analysis were as follows： there existed a moderate corralation between prostate volume and patients age （r= 0. 430, P〈0. 001）; there existed a moderate corralation between prostate volume and the degree of inflammation （r=0. 610 and r=0. 609, P〈0. 001） ; there existed a moderate corralation between PSA and the degree of inflammation （r=0. 572 and r=0. 487, P〈0.01） ; there was not significant correlation between PSA and patients age （r=0. 065, P〉0.1）; controlling influence of the age factor by partial correlation analysis, there was still a significant correlation between the prostate volume and level of inflammation. Conclusion： Most BPH patients are combined with prostatitis especially chronic inflammation. TNF-α and IL-6 are two pro-inflammatory cytokines, which obtain high exnression in the orostate tissue combined with histological inflammation. Patient＇s nrostate volume andPSA of BPH c