| 14-3-3蛋白在小鼠受精卯及2-细胞期胚胎中的表达与定位 |
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| 引用本文： | 崔城,于萌,张哲,于秉治.14-3-3蛋白在小鼠受精卯及2-细胞期胚胎中的表达与定位[J].生殖与避孕,2006,26(11):643-646. |
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| 作者姓名： | 崔城 于萌 张哲 于秉治 |
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| 作者单位： | 中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001 |
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| 摘 要： | 目的：研究小鼠受精卵及2-细胞期胚胎中14-3-3蛋白的表达及亚细胞定位。方法：采用Western blot方法鉴定小鼠受精卵和2-细胞期胚胎14-3-3蛋白的表达，利用间接免疫荧光技术观察其在细胞中的定位。结果：在小鼠受精卵和2-细胞期胚胎中，全部表达14-3-3蛋白，并且为同一亚型：14-3-3蛋白主要定位于受精卵的细胞质及2-细胞期胚胎中的细胞质和细胞核。结论：14-3-3蛋白正常表达于小鼠受精卵及2-细胞期胚胎中，其定位可能影响胚胎的早期发育。
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| 关 键 词： | 14-3-3蛋白 受精卵 二细胞期胚胎 定位 |
| 文章编号： | 0253-357X（2006）11-0643-04 |
| 收稿时间： | 2006-08-25 |
| 修稿时间： | 2006年8月25日 |
Expression and Localization of 14-3-3 Proteins in Mouse Fertilized Eggs and 2-cell Stage Embryos |
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| Cheng CUI, Meng YU, Zhe ZHANG, Bing-zhi YU.Expression and Localization of 14-3-3 Proteins in Mouse Fertilized Eggs and 2-cell Stage Embryos[J].Reproduction and Contraception,2006,26(11):643-646. |
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| Authors: | Cheng CUI Meng YU Zhe ZHANG Bing-zhi YU |
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| Institution: | Department of Biochemistry and Molecular Biology, School of Basic Medical Science China Medical University, Shenyang, 110001 |
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| Abstract: | Objective:To study the expression and localization of 14-3-3 proteins in mouse fertilized eggs and 2-cell stage embryos.Methods: Western blots and indirect immunofluorescence were performed to study the expression and localization of 14-3-3 proteins respectively.Results: The expression of 14-3-3 proteins was identified in mouse fertilized eggs and 2-cell stage embryos.In all phases of fertilized eggs investigated,14-3-3 proteins had a mainly cytoplasmic distribution and only high level of fluorescence was observed in one nucleus of the two blastomeres and moderate level of fluorescence of cytoplasm in 2-cell stage embryos.Conclusion: 14-3-3 proteins is not only expressed normally in mouse fertilized eggs and 2-cell stage embryos,but also its localization is likely to affect the development of mouse embryos. |
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| Keywords: | 14-3-3 proteins fertilized eggs 2-cell stage embryos localization |
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