溃疡性结肠炎大鼠结肠黏膜白细胞介素-6受体及白细胞介素-8受体的表达变化

目的探讨溃疡性结肠炎（UC）大鼠结肠黏膜白细胞介素-6（IL-6）受体及白细胞介素-8（IL-8）受体的表达变化。方法将雄性SD大鼠36只完全随机分为正常组、模型1组、模型2组共3组，每组12只。用5％2，4，6-三硝基苯磺酸100mg／kg灌肠建立大鼠溃疡性结肠炎模型，7d后处死模型1组动物，14d后处死模型2组动物。运用Real—Time—PCR法、ELISA法分别检测各组大鼠IL-6受体及IL-8受体mRNA及其含量的表达。结果模型1组、2组IL-6受体mRNA及含量表达分别为（3．35±0．12）pg／ml、（111．35±25．09）pg／ml及（3．56±0．09）pg/ml、（160．11±15．54）pg/ml；模型1组、2组IL-8受体mRNA及含量表达分别为（3．50±0．19）pg／ml、（160．11±15．54）pg／ml及（3．68±0．16）pg／ml、（185．38±15．42）pg／ml，与正常组相比，差异有统计学意义（P〈0．01）。结论活动性UC在炎症诱导下结肠组织中IL-6受体及IL-8受体mRNA及其含量表达增高，IL-6受体及IL-8受体的水平与肠道炎症程度呈正相关。

The expression and significance of IL - 6 receptor and IL - 8 receptor in a rat model of ulcerative colitis

Objective To discuss the expression and significance of IL- 6 receptor and IL- 8 receptor in a rat model of ulcerative colitis. Methods Thirty - six SD rats were randomly divided into 3 groups, twelve rats in each group, group 1 ： normal ; group 2 ： blank 1 ; group 3 ： blank 2. The rat model of ulcerative colitis was brought about by an enema of 100 mg/kg of TNBS and 5% ethanol. The blank 1 rat was sacrificed 1 week later. The other rats were sacrificed 2 weeks later. The expression of IL -6R and IL - 8R were detected with the technique of ELISA and Real - Time PCR. Results The mRNA and protein expression of IL - 6R were （3. 35 ± 0. 12） pg/ml, （111. 35 ± 25. 09） pg/ml and （3. 56± 0.09） pg/ml, （160.11 ± 15.54）pg/ml in blank 1 and blank 2. While the mRNA and protein expression of IL - 8 R were （ 3.50 ± 0.19 ） pg/ml, （ 160. 11 ±15.54 ） pg/ml and （ 3.68 ± 0.16 ） pg/ml, （ 185.38 ± 15.42） pg/ml respectively. Compared to normal group, there was a significant difference in the results of CINC - 1 and CXCR2 （ P 〈 0.01 ）. Conclusions The mRNA and protein expression of IL - 6R and IL - 8R is on the increment in active UC. There is relation between UC and the expression of IL - 6R and IL -8R.