大鼠心肌梗死后AChE阳性神经纤维再支配的观察

目的：观察大鼠心肌梗死后，梗死灶边缘区乙酰胆碱酯酶（AChE）阳性神经纤维密度的变化，探讨AChE阳性神经纤维的再支配过程。方法：实验用大鼠，结扎左冠状动脉前降支制备急性心肌梗死（AMI）模型，应用Kamovsky--Roots法和电镜组化技术，显示左心室梗死周围区1、2、12周AChE阳性神经纤维的密度变化。结果：①AMI后1、2周左心室梗死边缘区的AChE阳性神经纤维密度降低（P〈0．05），②12周后梗死边缘区的AChE阳性神经纤维密度升高，接近对照组。③电镜下见AChE阳性反应物以散在颗粒形式出现于肌间神经纤维走行处。结论：大鼠急性心肌梗死后12周，梗死周围区的存活心肌出现了神经再支配。

The experimental study on reinnervation of acetylcholinesterase positive nerves after acute myocardial infarction

Objective: To observe the reinnervation of acetylcholinesterase positive nerves(AChE) in the viable peri-infarct zones after acute myocardial infarction(AMI). Methods: The Wistar rats were used in this study. Left anterior decending coronary artery was ligated to form AMI. Hearts were excised 1, 2, 12 weeks after AMI. Karnovsky-Roots methods of histochemical techniques and electron microscopy were used to show the distribution of AChE positive nerves, while the density of AChE positive nerves was calculated by Medical Image Analysis Processing System. Results: The density of AChE positive nerves of viable peri-infarct zones decreased 1 and 2 weeks after AMI, however, the density of AChE positive nerves at same area increased obviously 12 weeks after AMI, and there were no statistical difference comparing it with control group. Under electron microscopy, AChE positive axons colored strongly showed scattered granules located within the intermuscular nerve fibers. Conclusions: Reinnervation appears in the viable peri-infarcted zone of myocardium 12 weeks after AMI.