HBV感染者血清HBcAg SP RIA测定的临床分析

目的:研究HBcAg在HBV感染者中的阳性检出率、分布规律及与其他乙肝标志物的关系,探讨其在反映HBV复制及传染性和观察疗效方面的临床价值.方法:采用SP RIA对461例HBV感染者进行血清乙肝六项指标测定,按其不同阳性结果分9种模式对比分析.结果:HBV感染者中HBcAg阳性总检出率达50.75%,而未感染者及69例抗-HBs单项阳性者中无阳性检出.结论:SP RIA测定血清HBcAg在HBV感染者中的阳性检出具有特异性,并可作为疑有抗-HBe阳性"逆转"为HBeAg阳性者的筛选检测,对判断HBV复制程度、病程、疗效及预后评估均有临床价值.

Clinical Analysis of Serum HBcAg Solid Phase RIA Result in HBV Infected Persons

Objective To suudy the HBcAg positive rate, distribution pattern in HBV infected persons and its correlation to other hepatitis B marker, also to evaluate its clinical value in reflecting HBV replication, infectivity and therapeutic effect. Methods Six serum hepatitis B markers (anti-HBc, anti-HBe, HBsAg, HBeAg, anti-HBs) in 461 cases HBV infected persons were detected by SP-radioimmunoassay (SPRIA) and nine combinations of various positive and negative markers were obtained for contrast analysis. Results HBcAg positive rate was 50.75% in HBV infected persons with none positive in 90 healthy controls and 69 patients with single anti-HBs positive only SP-RIA. Conclusions Serum HBcAg SP-RIA positive in HBV infected persons is of very high specificity and the assay could be useful in those HBeAg positives possibly reversed from anti-HBe positives to determine their present HBV replication activity, infectivity as well as to assess the therapeutic effect and prognosis.