| 1α,25二羟基维生素D_3对人早幼粒白血病细胞(HL—60)的诱导分化作用 |
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| 引用本文： | 薛延,王红霞,袁润英,相东,田洁,李秀森,魏文.1α,25二羟基维生素D_3对人早幼粒白血病细胞(HL—60)的诱导分化作用[J].中国药理学通报,1991(4). |
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| 作者姓名： | 薛延 王红霞 袁润英 相东 田洁 李秀森 魏文 |
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| 作者单位： | 北京积水潭医院北京市创伤骨科研究所生化研究室，北京积水潭医院北京市创伤骨科研究所免疫研究室，北京积水潭医院北京市创伤骨科研究所免疫研究室，北京积水潭医院北京市创伤骨科研究所免疫研究室，军事医学科学院基础医学研究所，军事医学科学院基础医学研究所，军事医学科学院基础医学研究所 100035，北京100850，北京100850，北京100850 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 1α,25二羟基维生素D_3在体外可诱导HL-60细胞向单核/巨噬样细胞发展。在形态上,核/质比例减少,核仁减少,细胞变得不规则并伸出伪足;在细胞化学方面,细胞对NBT还原能力增强,α萘酚醋酸酯酶和酸性磷酸酶阳性率明显增加,细胞周期的变化是:二倍体细胞明显增多,G_0+G_1期增加和S期减少。表明在1α,25(OH)_2D_3作用下,HL-60细胞向成熟分化,细胞DNA合成受抑制。
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| 关 键 词： | 1α 25二羟基维生素D_3 诱导分化 人早幼粒白血病细胞 细胞周期 |
EFFECT OF 1α, 25-DIHYDROXY-VITAMIN D_3 ON DIFFERENTIATION AND PROLIFERATION OF A HUMAN MYELOID LEUKEMIA CELL LINE |
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| Xue Yan,Tian Jie,Wang Hongxia et al.EFFECT OF 1α, 25-DIHYDROXY-VITAMIN D_3 ON DIFFERENTIATION AND PROLIFERATION OF A HUMAN MYELOID LEUKEMIA CELL LINE[J].Chinese Pharmacological Bulletin,1991(4). |
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| Authors: | Xue Yan Tian Jie Wang Hongxia |
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| Abstract: | 1 α 25-dihydroxy-vitamin D3(1α 25 (OH)2 D3)induced differentiation of human promyelocytic leukaemia cells ( HL-60 ) into mature myeloid cells in vitro. The ratio of nuclei to cytoplasma decreased; Their nucleoli reduced; Cells became irrgular and extended the pseudopods. 1 α 25 (OH ) 2 D3 caused significant increase of nitroblue tetrazolium ( NBT ) reduction and α-non-specific acid esterase (α-NAE ) and acid phosphatase (ACP ) activities. These data and morphological characteristics suggest that HL-60 cells were conclusively identified as monocytes/macrophages. The histogram of DNA distribution ahylyzed by flow cytometry demonstrated G0 + G1 phase increased and S phase increased and S phase decreased remarkably after treatment with lα, 25 ( OH ) 2 D3 as compared with untreated cells. |
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| Keywords: | 1 a 25-Dihydroxy-vitamin D3 Induced differentiation Human myetoid leukaemia cell line |
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