HPLC法测定注射用舒血宁中总黄酮醇苷的含量

目的建立以高效液相色谱法测定注射用舒血宁中总黄酮醇苷含量的方法。方法色谱柱为Dikma DiamonsilTM C18(200mm×4.6mm,5μm),流动相为甲醇-0.5%磷酸溶液(54∶46),流速为1.0mL.min-1,检测波长为360nm,柱温为25℃。结果总黄酮醇苷中槲皮素在0.0071~0.0852mg·mL-1(r=0.9990,n=5)、山柰素在0.0065~0.0780mg·mL-1(r=0.9997,n=5)、异鼠李素在0.0015~0.0180mg·mL-1(r=0.9981,n=5)浓度范围内具有较好的线性关系;平均加样回收率总黄酮醇苷为101.2%(RSD=0.5%),槲皮素为99.2%(RSD=0.9%),山柰素为103.8%(RSD=0.8%),异鼠李素为102.7%(RSD=2.0%)。结论本方法测定结果准确、操作简便、灵敏度高、重现性好,可用于本品的质量控制。

Determination of Total Favonol Glycosides in Shuxuening for Injection by HPLC

OBJECTIVE: To establish an HPLC method for determination of the content of Total Favonol Glycosides in Shuxuening for Injection. METHODS: Dikma DiamonsilTM C18( 200mm× 4. 6mm, 5μ m) column was used as chromatographic column, and methanol- 0. 5% acid water ( 54∶ 46) as mobile phase. The detection wavelength was set at 360nm, flow rate at 1. 0mL· min- 1, and column temperature at 25℃ . RESULTS: There was a good linear relationship when Quercetin of Total Favonol Glycosides was in the range of 0. 007 1～ 0. 085 2mg· mL- 1( r=0. 999 0, n=5) , Kaempferol in the range of 0. 006 5～ 0. 078 0mg· mL- 1( r=0. 999 7, n=5) , and Isorhamnetin in the range of 0. 001 5～ 0. 018 0mg· mL- 1( r=0. 998 1, n=5) . The average recoveries of Total Favonol Glycosides, Quercetin, Kaempferol and Isorhamnetin were respectively 101. 2% ( RSD=0. 5% ) , 99. 2% ( RSD=0. 9% ) , 103. 8% ( RSD=0. 8% ) and 102. 7% ( RSD=2. 0% ) . CONCLUSIONS: This method is accurate in determination results, simple in operation, high in sensitivity and good in reproducibility, and can be used for the quality control of the preparation.