| 热休克蛋白90α的表达及其对体内肿瘤生长的影响 |
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| 引用本文： | 乔绘红,赵玫,袁兴华. 热休克蛋白90α的表达及其对体内肿瘤生长的影响[J]. 中华肿瘤杂志, 1999, 0(1): 19-21 |
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| 作者姓名： | 乔绘红 赵玫 袁兴华 |
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| 作者单位： | 中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院病因及癌变研究室 |
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| 基金项目： | 卫生部科研基金,中国医学科学院研究基金 |
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| 摘 要： | 目的 探讨ｈｓｐ９０α对体内肿瘤生长的影响及机理。方法 利用克隆技术构建了ｈｓｐ９０α融合表达质粒ｐＭａｌ－ｈｓｐ９０α。ＤＢＡ／２小鼠接种肿瘤细胞前，皮下注射融合蛋白，每隔１天测肿瘤大小，２５天后，＾５１Ｃｒ释放法测ＮＫ细胞活性。结果 注射热休克帽白组小鼠抗ｈｓｐ９０ａ抗体升高，肿瘤生长快，ＮＫ细胞活性低，结论 ｈｓｐ９０α对肿瘤生长的影响与不鼠体内的ＮＫ细胞活性降低有关。
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| 关 键 词： | 热休克蛋白类 基因表达 肿瘤 免疫学 NK细胞 |
Expression of heat shock protein 90 alpha and its effect on the growth of tumor in mice] |
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| QIAO Huihong,ZHAO Mei,YUAN Xinghua,et al.. Expression of heat shock protein 90 alpha and its effect on the growth of tumor in mice][J]. Chinese Journal of Oncology, 1999, 0(1): 19-21 |
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| Authors: | QIAO Huihong ZHAO Mei YUAN Xinghua et al. |
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| Affiliation: | Cancer Institute, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021. |
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| Abstract: | OBJECTIVE: To study the effect of hsp90 alpha on the growth of tumor in mice and its mechanism. METHODS: Hsp90 alpha fusion protein was expressed in E. coli. DBA/2 mice were injected with purified fusion protein, then challenged with L1210 cells. The size of tumor was measured every other day. NK cell activity was detected by 51Cr release assay. RESULTS: The tumors grew faster while activity NK cell activity of mice was lower in the test mice than that in the control mice. CONCLUSION: The results suggest that the accelerated tumor growth is probably due to the formation of anti-hsp90 alpha antibody and decreased NK cell activity of mice treated with hsp90 alpha. |
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| Keywords: | Heat shock proteins Gene expression Neoplasms/immunology Killer cells natural L 1210 cell line |
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