食管癌发生发展过程中GST-π基因表达的研究

目的 探讨ＧＳＴ－π基因在食管癌发生过程中的作用。方法 采用４８例食管鳞癌标本建立食管癌发生发展多阶段模型，应用免疫组化和原位杂交方法对食管癌发生发展过程中ＧＳＴ－基因表达进行研究。结果在正常粘膜，单纯增生，不典型增生上皮总体及其Ⅰ、Ⅱ、Ⅲ级，原位癌和癌组织中ＧＳＴ－π蛋白表达阳性率分别为８７．５％，９５．３％，５５．９％，７３．９％，４７．４％，４１．２％、３６．４％和４５．８％．正常粘膜和单纯

Expression of GST-pi gene in human esophageal carcinogenesis]

OBJECTIVE: To investigate the possible role of GST-pi in esophageal carcinogenesis. METHODS: GST-pi expression at mRNA level was studied by in situ hybridization (ISH) and at protein level by immunohistochemistry (IHC). GST-pi expression of the esophagus in normal epithelial cells (NC), hyperplastic cells (HC), dysplastic cells (DC) from grade I to III, carcinoma in-situ (CIS) and invasive carcinoma (IC) was examined in the same esophageal cancer specimens (n = 48) which provided a model reflecting the process of esophageal carcinogenesis. RESULTS: The positive rate of IHC staining was 87.5% for NC, 95.3% for HC, 55.9% for DC (grade I: 73.9%, grade II: 47.4%, grade III: 41.2%), 36.4% for CIS and 45.8% for IC. The positive rate of GST-pi mRNA expression was 81.2% for NC, 94.4% for HC, 61.9% for DC (grade I: 76.5%, grade II: 61.5%, grade III: 41.7%), 44.4% for CIS and 83.3% for grade I IC, 30.0% for grade II IC and 0% for grade III IC. There was no statistically significant difference in GST-pi expression at the mRNA and the protein level. CONCLUSION: There is a decreasing tendency of GST-pi expression from dysplasia to CIS and IC. The decrease in GST-pi expression is an early event in esophageal carcinogenesis.