姜黄素对大鼠视网膜缺血再灌注后细胞凋亡及p53表达的影响

目的 探讨姜黄素对大鼠视网膜缺血再灌注后细胞凋亡及凋亡相关基因p53表达的影响。方法制作大鼠视网膜缺血再灌注模型，并随机分为假手术组（SH组）、缺血再灌注组（IR组）、姜黄素处理组（CU组）和对照组（SC组），分别于再灌注后2、6、24、72h和7d时观察大鼠视网膜和视网膜毛细血管细胞的凋亡情况，并检测视网膜Caspase-3酶的表达活性及p53基因的表达情况。结果各组模型均制作成功，细胞组织学检查可见CU组损伤明显轻于其他组。CU组视网膜和视网膜毛细血管的细胞凋亡数量均显著多于SH组（均P〈0.01），但显著少于IR组（P〈0．01）；CU组p53表达水平及Caspase-3酶活性均显著强于SH组（均P〈0.01），但显著弱于IR组（P〈0．01）。结论姜黄素对大鼠视网膜缺血再灌注损伤具有保护作用，调控凋亡相关基因p53蛋白的表达可能是其作用机制之一。

The effect of curcumin on apoptosis and the expression of p53 in rat retina after ischemia-reperfusion

Objective To investigate the effect of curcumin on apoptosis and the expression of p53 in rat retina after retina ischermia reperfusion （RIR）. Methods Male Wistar-Kyoto rats （WKY） were randomly divided into sham group （SH） , ischemia reperfusion group（IR）, curcumin group （CU） and solvent control group （SC）,and each group was subdivided into 2 h, 6 h, 24 h, 72 h and 7d after reperfusion （n=9 in each）. Retina and the retinal capillary apoptosis were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） methods and the caspase-3 activity was evaluated by spectrophotometric analysis. The expression of p53 was determined by streptavidin-perosidase（ SP）immunohistochemistry. Results The degree of apoptosis in retina and the retinal capillary were milder in CU group than that in IR group （ P 〈 0.01 ）. The expression of p53 and the activity of caspase-3 was down-regulated in retina of CU group than that of IR group （ P 〈 0.01 ）. Conclusion Curcumin can protect retina and retinal capillary from RIR, which may.be associated with inhibition of p53-related apoptosis.