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| 黄芪对柯萨奇B_3病毒性心肌炎小鼠单核细胞趋化蛋白-1表达的影响 | |
| 引用本文： | 蔡珊珊,黄星原,王世红,陈莉琼,郭海鹏,黄从新.黄芪对柯萨奇B_3病毒性心肌炎小鼠单核细胞趋化蛋白-1表达的影响[J].武汉大学学报(医学版),2009,30(3). |
| 作者姓名： | 蔡珊珊 黄星原 王世红 陈莉琼 郭海鹏 黄从新 |
| 作者单位： | 1. 武汉大学人民医院儿科武汉市儿童医院,湖北,武汉,430015 2. 武汉大学人民医院儿科,湖北,武汉,430060 3. 武汉大学人民医院心内科,湖北,武汉,430060 |
| 基金项目： | 湖北省科技攻关项目(编号:2003AA301C21) |
| 摘 要： | 目的:探讨黄芪对柯萨奇B3(CVB3)病毒性心肌炎小鼠单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:55只BALB/c小鼠,雄性,周龄为6-8周,随机分成3组:A组为空白对照组(15只);B组为病毒对照组(20只);C组为黄芪治疗组(20只)。于接种病毒第14天处死所有存活小鼠,取心脏,分别用光镜检查心肌组织的病理变化,用免疫组化方法和逆转录-聚合酶链反应(RT-PCR)检测心肌组织中MCP-1的表达。结果:①黄芪治疗组生存率较病毒对照组提高(P<0.05);②病毒对照组与空白对照组相比心肌组织中可见大量的MCP-1的表达,且于心肌的病变面积成正比(P<0.01,r=0.87);③黄芪治疗组心肌病理积分和MCP-1的表达明显低于病毒对照组(P<0.01)。结论:黄芪可以减少病毒性心肌炎心肌组织MCP-1的产生可能是其治疗病毒性心肌炎的机制之一。 |
| 关 键 词： | 柯萨奇B3病毒 病毒性心肌炎 MCP-1 黄芪 |
Effect of Astragalus Mongholicus on the Expression of Monocyte Chemoattractant Protein-1 in Acute Coxsackievirus B_3 Murine Myocarditis |
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| CAI Shanshan,HUANG Xingyuan,WANG Shihong,CHEN Liqiong,GUO Haipeng,HUANG Congxin.Effect of Astragalus Mongholicus on the Expression of Monocyte Chemoattractant Protein-1 in Acute Coxsackievirus B_3 Murine Myocarditis[J].Medical Journal of Wuhan University,2009,30(3). | |
| Authors: | CAI Shanshan HUANG Xingyuan WANG Shihong CHEN Liqiong GUO Haipeng HUANG Congxin |
| Institution: | CAI Shanshan1,2,HUANG Xingyuan1,WANG Shihong1,CHEN Liqiong1,GUO Haipeng3,HUANG Congxin3 1Dept.of Pediatrics,3Dept.of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,China2Children's Hospital of Wuhan,Wuhan 430015,China |
| Abstract: | Objective: To investigate the effect of Astragalus mongholicus on the expression of monocyte chemoattractant protein-1(MCP-1) in acute Coxsackievirus B3(CVB3) murine myocarditis.Methods: A total of 55 BALB/c mice were assigned into three groups: normal control group(group A,n=15),CVB3 infected group(group B,n=20),and Astragalus mongholicus treated group(group C,n=20).The mice in group B and C were inoculated with 0.15 ml of TCID50 10-9/L CVB3,while mice in group A were inoculated with 0.15 ml of Eagle's rea... |
| Keywords: | Coxsackievirus B3 Viral Myocarditis Monocyte Chemoattractant Protein-1 Astragalus Mongholicus |
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