细胞内砷浓度与三氧化二砷治疗敏感性的关系

 目的 研究几种白血病和实体瘤细胞内三氧化二砷（As₂O₃）浓度对其治疗敏感性的影响。方法 将几种白血病细胞株和原代细胞及实体瘤细胞株分别给予特定浓度和时间As₂O₃干预后，原子荧光法测定细胞内砷浓度。流式细胞仪检测细胞凋亡率。结果 一定浓度和时间As₂O₃干预后，不同细胞内药物浓度不同。NB4细胞株＞APL原代＞K562细胞株＞CML原代＞HL60细胞株＞AML-M2原代＞HeLa细胞株＞肝癌腹水H₂₂细胞株＞胃腺癌SGC-7901细胞株＞喉癌Hep-2细胞株。在最低促凋亡浓度水平以上的低浓度、长时间持续干预比短期高浓度干预的细胞内砷浓度高，凋亡率大。结论 细胞内砷浓度不同可能是As₂O₃治疗敏感性不同的原因之一。

Relationship between intracellular arsenic concentration and arsenic trioxide therapeutic sensitivity

OBJECTIVE To study the relationship between intracellular concentrations of arsenic trioxide (As 2O 3) in several kinds of leukemia or solid carcinoma cell strains and the therapeutic sensitivities. METHODS Intracellular arsenic concentrations were assayed with atom fluorescent method. Apoptosis rate was evaluated with flow cytometry. RESULTS After being managed by As 2O 3 of different concentrations and for different durations, different cell strains had different intracellular arsenic concentrations. The sequence was as follows: NB4 cell line>APL primary cell>K562 cell line >CML primary cell>HL-60 cell line>AML-M2 primary cell >HeLa cell line> H-22 cell line>SGC-7901 cell line >cancer of larynx Hep-2 cell line. There were higher intracellular arsenic concentrations in NB4, K562 and APL cell strains cultured with As 2O 3 of lower concentrations above the lowest apoptotic concentration for a long time than with As 2O 3 of higher concentration for a short period. CONCLUSION Different leukemic and solid carcinoma cell strains had different intracellular arsenic concentrations, which might be one of the factors that determined the sensitivities of leukemia and solid carcinoma to As 2O 3 in clinical treatment.